HDAC3/NCOR2 recombinant protein

Recombinant HDAC3/NCOR2 complex, His-Tag

Cat. Num. AAA388306

• Gene Names: HDAC3; HD3; RPD3; RPD3-2
• Synonyms: HDAC3/NCOR2; Recombinant HDAC3/NCOR2 complex; His-Tag; Histone Deacetylase 3; HD3; SMAP45; HDAC3/NCOR2 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant HDAC3/NCOR2 Complex, His-tag is supplied in 50mM Tris-HCl pH8.0, 300mM NaCl, 5% glycerol.

• Protein Species: Human
• Tag: GST-Tag, His-Tag
• Notes: This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. HDAC Activity Assay Conditions: 3uM H3K9ac peptide was incubated with different concentrations of HDAC3/NCOR2 Complex, His-tag in reaction buffer including 25mM Tris-HCl pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2, 0.1mg/ml BSA for 30 minutes at 37 degree C. HTRF assay was used for activity detection.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant HDAC3 / NCOR2 complex, His-tag 12% SDS-PAGE Coomassie staining MW: HDAC3: 49.7 kDa MW: NCOR2: 37.9 kDa Purity: > 85%)

Testing Data

(HTRF assay for HDAC3 / NCOR2 Complex, His-tag activity 3 uM histone H3K9ac peptide was incubated with different concentrations of HDAC3 / NCOR2 Complex, His-tag in reaction buffer for 30 minutes at 37 degree C. H3K9me0-Eu antibody was used to detect the reaction products.)

Testing Data

(HTRF assay for HDAC3 / NCOR2 Complex, His-tag activity 3 uM histone H3K9ac peptide was incubated with different concentrations of HDAC3 / NCOR2 Complex, His-tag in reaction buffer for 30 minutes at 37 degree C. H3K9me0-Eu antibody was used to detect the reaction products.)

Related Product Information for HDAC3/NCOR2 recombinant protein

Short Description: Recombinant HDAC3/NCOR2 complex, His-tag includes full-length human HDAC3 protein (accession number NP_003874.2) with a C-terminal 6xHis-tag and human NCOR2 amino acids 395-489 (accession number NP_006303.4) with an N-terminal GST-Tag; it was expressed in Sf9 cells. The molecular weights of HDAC3 and NCOR2 (395-489) are 49.7 kDa and 37.9 kDa, respectively. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: HDAC3 (Histone Deacetylase 3, also designated HD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11). By forming multi-protein complexes with the co-repressors NCOR2, HDAC3 regulates the transcription of a plethora of genes. A growing list of non-histone proteins extends the role of HDAC3 beyond transcriptional repression. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

Product Categories/Family for HDAC3/NCOR2 recombinant protein

Recombinant Proteins; Histone Deacetylase (HDAC)

NCBI and Uniprot Product Information

• NCBI GI #: 13128862
• NCBI GeneID: 8841
• NCBI Accession #: NP_003874.2; NP_006303.4
• NCBI GenBank Nucleotide #: NM_003883.3
• UniProt Accession #: O15379; O43268; Q9UEI5; Q9UEV0; D3DQE1
• Molecular Weight: 48,848 Da
• NCBI Official Full Name: histone deacetylase 3
• NCBI Official Synonym Full Names: histone deacetylase 3
• NCBI Official Symbol: HDAC3
• NCBI Official Synonym Symbols: HD3; RPD3; RPD3-2
• NCBI Protein Information: histone deacetylase 3; SMAP45
• UniProt Protein Name: Histone deacetylase 3
• UniProt Gene Name: HDAC3
• UniProt Synonym Gene Names: HD3
• UniProt Entry Name: HDAC3_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC3: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1, PRDM6 and SRY. Interacts with BTBD14B. Interacts with GLIS2. Interacts (via the DNA-binding domain) with NR2C1; the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Component of the Notch corepressor complex. Interacts with CBFA2T3 and NKAP. Interacts with APEX1; the interaction is not dependent on the acetylated status of APEX1. Interacts with and deacetylates MAPK14. Interacts with ZMYND15. Widely expressed. Belongs to the histone deacetylase family. HD type 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Apoptosis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Deacetylase; EC 3.5.1.98

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; Golgi apparatus; transcriptional repressor complex; spindle microtubule; cytoplasm; histone deacetylase complex; plasma membrane; nucleus

Molecular Function: protein binding; cyclin binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); chromatin DNA binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription factor binding; transcription corepressor activity

Biological Process: circadian rhythm; negative regulation of JNK cascade; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; transcription, DNA-dependent; nerve growth factor receptor signaling pathway; regulation of mitotic cell cycle; regulation of multicellular organism growth; regulation of protein stability; chromatin modification; spindle assembly; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell cycle; protein amino acid deacetylation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; positive regulation of TOR signaling pathway; negative regulation of apoptosis