SDS-Page
(Recombinant HDAC1 protein gel. HDAC1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 56 kDa Purity> >65%)
HDAC1 recombinant protein
Recombinant HDAC1 protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant HDAC1 protein gel. HDAC1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 56 kDa Purity> >65%)
SDS-Page
(Recombinant HDAC1 protein gel. HDAC1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 56 kDa Purity> >65%)
Testing Data
(Recombinant HDAC1 activity by HTRF Assay. 1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant HDAC1 activity by HTRF Assay. 1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant HDAC1 activity by HTRF Assay. 1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant HDAC1 activity by HTRF Assay. 1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of HDAC1 protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Background: HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11). HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.
NCBI and Uniprot Product Information
NCBI Description
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
Uniprot Description
HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Protein type: Hydrolase; Nuclear receptor co-regulator; EC 3.5.1.98
Chromosomal Location of Human Ortholog: 1p34
Cellular Component: nucleoplasm; protein complex; Sin3 complex; cytoplasm; histone deacetylase complex; nuclear chromatin; NuRD complex; chromatin; nucleus; cytosol
Molecular Function: deacetylase activity; nucleosomal DNA binding; transcription activator binding; histone deacetylase binding; protein deacetylase activity; protein N-terminus binding; transcription factor binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor activity
Biological Process: establishment and/or maintenance of chromatin architecture; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; protein amino acid deacetylation; negative regulation of gene expression, epigenetic; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; circadian regulation of gene expression; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; histone deacetylation; chromatin modification; odontogenesis of dentine-containing teeth; chromatin remodeling; ATP-dependent chromatin remodeling; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; blood coagulation; negative regulation of apoptosis
Research Articles on HDAC1
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Product Notes
The HDAC1 hdac1 (Catalog #AAA388214) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HDAC1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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