SDS-Page
(Recombinant HDAC8 His-Tag protein gel. HDAC8 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
HDAC8 recombinant protein
Recombinant HDAC8 protein, His-Tag
Shipping Temp: Dry Ice
SDS-Page
(Recombinant HDAC8 His-Tag protein gel. HDAC8 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant HDAC8 His-Tag protein gel. HDAC8 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
Testing Data
(HTRF assay for HDAC8 protein, His-Tag 3 uM Histone H3K9ac (1-21aa) peptide was incubated with HDAC8 protein, His-Tag in reaction buffer for 30 min at 37 degree C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.)
Testing Data
(HTRF assay for HDAC8 protein, His-Tag 3 uM Histone H3K9ac (1-21aa) peptide was incubated with HDAC8 protein, His-Tag in reaction buffer for 30 min at 37 degree C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.)
Testing Data
(HTRF assay for HDAC8 protein, His-Tag 3 uM Histone H3K9ac (1-21aa) peptide was incubated with HDAC8 protein, His-Tag in reaction buffer for 30 min at 37 degree C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.)
Testing Data
(HTRF assay for HDAC8 protein, His-Tag 3 uM Histone H3K9ac (1-21aa) peptide was incubated with HDAC8 protein, His-Tag in reaction buffer for 30 min at 37 degree C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.)
Background: HDAC8 (Histone Deacetylase 8) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC8 catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. It also is involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. HDAC8 may play a role in smooth muscle cell contractility.
NCBI and Uniprot Product Information
NCBI Description
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Uniprot Description
HDAC8: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. Belongs to the histone deacetylase family. HD type 1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.5.1.98; Hydrolase
Chromosomal Location of Human Ortholog: Xq13
Cellular Component: nucleoplasm; nuclear chromosome; histone deacetylase complex; cytoplasm; plasma membrane; cytosol; nucleus
Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor binding
Biological Process: chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; sister chromatid cohesion; mitotic cell cycle; chromatin modification; negative regulation of transcription from RNA polymerase II promoter
Disease: Wilson-turner X-linked Mental Retardation Syndrome
Research Articles on HDAC8
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Product Notes
The HDAC8 hdac8 (Catalog #AAA388269) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HDAC8, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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