Mouse anti-Human Sex Hormone Binding Globulin (SHBG) Monoclonal Antibody | anti-SHBG antibody

Sex Hormone Binding Globulin (SHBG) Mouse Monoclonal Antibody

Cat. Num. AAA439054

• Gene Names: SHBG; ABP; SBP; TEBG
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Sex Hormone Binding Globulin (SHBG); Monoclonal Antibody; Sex Hormone Binding Globulin (SHBG) Mouse Monoclonal Antibody; Androgen binding protein (ABP); Sex hormone-binding globulin (SHBG); Sex steroid binding protein (SBP); Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone binding beta globulin; Testosterone-Estradiol-Binding Globulin (TEBG); anti-SHBG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SPM605
• Specificity: Recognizes a protein of 45kDa, identified as SHBG. It functions as an androgen transport protein, but may also be involved in receptor-mediated processes. Each dimer binds one molecule of steroid. It is specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. SHBG regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 402

• Applicable Applications for anti-SHBG antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic & Extracellular (Secreted)
• Immunogen: Recombinant full-length human SHBG protein
• Hu-Chromosome Location: 17p13.1
• Positive Control: Liver, Placenta or Testis
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with SHBG Monoclonal Antibody (SPM605))

References

Grishkovskaya, I., et al. 2000. Crystal structure of human sex hormone-binding globulin: steroid transport by a laminin G-like domain. EMBO. J. 19: 504-512.

NCBI and Uniprot Product Information

• NCBI GI #: 7382460
• NCBI GeneID: 6462
• NCBI Accession #: NP_001031.2
• NCBI GenBank Nucleotide #: NM_001040.4
• UniProt Accession #: P04278; P14689; Q16616; Q3MIL0; Q6ISD2; B0FWH4; E9PGW1; F5H5Z8; I3L1N7
• Molecular Weight: 45kDa
• NCBI Official Full Name: sex hormone-binding globulin isoform 1
• NCBI Official Synonym Full Names: sex hormone binding globulin
• NCBI Official Symbol: SHBG
• NCBI Official Synonym Symbols: ABP; SBP; TEBG
• NCBI Protein Information: sex hormone-binding globulin
• UniProt Protein Name: Sex hormone-binding globulin
• Protein Family: Sex hormone-binding globulin
• UniProt Gene Name: SHBG
• UniProt Synonym Gene Names: SHBG; SBP; ABP; TeBG
• UniProt Entry Name: SHBG_HUMAN

NCBI Description

This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

SHBG: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13.1

Molecular Function: androgen binding