HDAC5 recombinant protein

Recombinant HDAC5 (656-1122) protein

Cat. Num. AAA388244

• Gene Names: HDAC5; HD5; NY-CO-9
• Purity: The protein is >90% pure by SDS-PAGE.
• Synonyms: HDAC5; Recombinant HDAC5 (656-1122) protein; HDAC-5; Histone Deacetylase 5; HD5; HDAC5 recombinant protein

• Host: Baculovirus
• Purity/Purification: The protein is >90% pure by SDS-PAGE.
• Form/Format: Recombinant HDAC5 (656-1122) protein is supplied in 25mM HEPES pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: Recombinant HDAC5 protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant HDAC5 protein gel.HDAC5 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)

Testing Data

(HDAC-Glo Class IIa Assay for HDAC5 activityAssay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 uM substrate was incubated with HDAC5 proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)

Testing Data

(HDAC-Glo Class IIa Assay for HDAC5 activityAssay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 uM substrate was incubated with HDAC5 proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)

Related Product Information for HDAC5 recombinant protein

Short Description: Recombinant HDAC5 (656-1122) protein includes amino acids 656-1122 of human HDAC5 protein (accession number NP_005465.2); it was expressed in Sf9 cells and contains an N-terminal DYKDDDDK-Tag with a molecular weight of 53.1 kDa. The protein is >90% pure by SDS-PAGE. It is suitable for analysis of lysine deacetylation of histones and other cellular proteins, as well as for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: HDAC5 (Histone Deacetylase 5) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue.In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).Like HDAC4, HDAC5 shuttles between the nucleus and cytoplasm. In muscle cells, this protein shuttles into the cytoplasm during myocyte differentiation. HDAC5 interacts with many different transcription factors including HDAC3, and may form multi-complex proteins.

Product Categories/Family for HDAC5 recombinant protein

Recombinant Proteins; Histone Deacetylase (HDAC)

NCBI and Uniprot Product Information

• NCBI GI #: 62750347
• NCBI GeneID: 10014
• NCBI Accession #: NP_005465.2
• NCBI GenBank Nucleotide #: NM_005474.4
• UniProt Accession #: Q9UQL6; O60340; O60528; Q96DY4; C9JFV9
• Molecular Weight: 122,049 Da
• NCBI Official Full Name: histone deacetylase 5 isoform 1
• NCBI Official Synonym Full Names: histone deacetylase 5
• NCBI Official Symbol: HDAC5
• NCBI Official Synonym Symbols: HD5; NY-CO-9
• NCBI Protein Information: histone deacetylase 5; antigen NY-CO-9
• UniProt Protein Name: Histone deacetylase 5
• UniProt Gene Name: HDAC5
• UniProt Synonym Gene Names: KIAA0600; HD5
• UniProt Entry Name: HDAC5_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC5: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Coimmunoprecipitates only with HDAC3 family members. Interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. Two alternatively spliced isoforms have been described.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; nuclear body; Golgi apparatus; histone deacetylase complex; cytoplasm; nucleus; cytosol

Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; protein kinase C binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; metal ion binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity; transcription factor binding

Biological Process: establishment and/or maintenance of chromatin architecture; heart development; chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; protein amino acid deacetylation; negative regulation of osteoblast differentiation; inflammatory response; response to drug; Notch signaling pathway; regulation of skeletal muscle fiber development; transcription, DNA-dependent; B cell activation; histone deacetylation; chromatin modification; response to cocaine; osteoblast development; chromatin remodeling; cellular response to insulin stimulus; regulation of protein binding; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; multicellular organismal response to stress