HDAC10 recombinant protein

Recombinant HDAC10 (2-631) protein

Cat. Num. AAA389357

• Gene Names: HDAC10; HD10
• Synonyms: HDAC10; Recombinant HDAC10 (2-631) protein; Histone Deacetylase 10; HDAC10 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant HDAC10 (2-631) protein is supplied in 25mM HEPESNaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant HDAC10 (2-631) protein gel 10% SDS-PAGE with Coomassie blue staining MW: 68.4 kDa Purity: >70%)

Related Product Information for HDAC10 recombinant protein

Short Description: Recombinant HDAC10 (2-631) protein that includes amino acid 2-631 of human HDAC10 (accession number NP_114408.3) was expressed in a baculovirus expression system with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 68.4 kDa.

Background: HDAC10 (Histone Deacetylase 10) is a member of the class II mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones or many cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e -amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC10 is a polyamine deacetylase (PDAC), which acts preferentially on N(8)-acetylspermidine, and also on acetylcadaverine and acetylputrescine. It exhibits attenuated catalytic activity toward N(1), N(8)-diacetylspermidine and very low activity, if any, toward N(1)-acetylspermidine. HDAC10 has also been shown to be involved in MSH2 deacetylation. The physiological relevance of protein/histone deacetylase activity is unclear and could be very weak.

Product Categories/Family for HDAC10 recombinant protein

Recombinant Proteins; Histone Deacetylase (HDAC)

NCBI and Uniprot Product Information

• NCBI GI #: 20070354
• NCBI GeneID: 83933
• NCBI Accession #: NP_114408.3
• NCBI GenBank Nucleotide #: NM_032019.5
• UniProt Accession #: Q969S8; Q08AP4; Q6STF9; Q96P77; Q96P78; Q9H028; Q9UGX1; Q9UGX2
• Molecular Weight: 69,385 Da
• NCBI Official Full Name: histone deacetylase 10 isoform 1
• NCBI Official Synonym Full Names: histone deacetylase 10
• NCBI Official Symbol: HDAC10
• NCBI Official Synonym Symbols: HD10
• NCBI Protein Information: histone deacetylase 10
• UniProt Protein Name: Histone deacetylase 10
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC10
• UniProt Synonym Gene Names: HD10
• UniProt Entry Name: HDA10_HUMAN

NCBI Description

The protein encoded by this gene belongs to the histone deacetylase family, members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure, and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

HDAC10: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Belongs to the histone deacetylase family. HD type 2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; EC 3.5.1.98; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; histone deacetylase complex; cytoplasm; nucleus

Molecular Function: protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity

Biological Process: Notch signaling pathway; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; protein amino acid deacetylation; regulation of transcription, DNA-dependent; histone deacetylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; oligodendrocyte development; negative regulation of transcription, DNA-dependent