HDAC11 recombinant protein

Recombinant HDAC11 (2-347) protein

Cat. Num. AAA388242

• Gene Names: HDAC11; HD11
• Purity: The purity of HDAC11 protein is >70% by SDS-PAGE.
• Synonyms: HDAC11; Recombinant HDAC11 (2-347) protein; HDAC-11; Histone Deacetylase 11; HD11; HDAC11 recombinant protein

• Host: Baculovirus
• Purity/Purification: The purity of HDAC11 protein is >70% by SDS-PAGE.
• Form/Format: Recombinant HDAC11 (2-347) protein is supplied at a concentration of 0.15ug/ul in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: Recombinant HDAC11 protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. HDAC Activity Assay Conditions: 1uM H3K9ac peptide was incubated with different concentrations of HDAC11 protein in a 10ul reaction system containing 25mM Tris-HCl pH 8.0, 137mM NaCl, 2.7mM MgCl2, 1mM KCl and 0.1mg/ml BSA for 30 minutes. 10ul H3K9ac0 antibody and SA-CL665 mixture (each at 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All reactions were performed at room temperature. HTRF assay was used for detection.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant HDAC11 (2-347) protein gel. HDAC11 (2-347) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 42.2 kDa Purity: > 70%)

Testing Data

(HTRF assay for HDAC11 (2-347) protein activity 1 uM histone peptide H3K9ac (1-21) was incubated with HDAC11 (2-347) in reaction buffer for 30 minutes at 37 degree C. H3K9me0 antibody was used to detect reaction products.)

Testing Data

(HTRF assay for HDAC11 (2-347) protein activity 1 uM histone peptide H3K9ac (1-21) was incubated with HDAC11 (2-347) in reaction buffer for 30 minutes at 37 degree C. H3K9me0 antibody was used to detect reaction products.)

Related Product Information for HDAC11 recombinant protein

Short Description: Recombinant HDAC11 (2-347) protein includes amino acids 2-347 of human HDAC11 protein (accession number NP_079103.2); it was expressed in Sf9 cells and contains an N-terminal DYKDDDDK-Tag with a molecular weight of 42.2 kDa. The purity of HDAC11 protein is >70% by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: HDAC11 (Histone Deacetylase 11) is a member of the class IV mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue.In humans, expression of HDAC11 is limited to only a few organs, including brain, heart, muscle and kidney, suggesting an important role of HDAC11 in those tissues.

Product Categories/Family for HDAC11 recombinant protein

Recombinant Proteins; Histone Deacetylase (HDAC)

NCBI and Uniprot Product Information

• NCBI GI #: 217272877
• NCBI GeneID: 79885
• NCBI Accession #: NP_079103.2
• NCBI GenBank Nucleotide #: NM_024827.3
• UniProt Accession #: Q96DB2; Q9H6I7; Q9H6X3; Q9NTC9; B4DDK1
• Molecular Weight: 33,124 Da
• NCBI Official Full Name: histone deacetylase 11 isoform 1
• NCBI Official Synonym Full Names: histone deacetylase 11
• NCBI Official Symbol: HDAC11
• NCBI Official Synonym Symbols: HD11
• NCBI Protein Information: histone deacetylase 11
• UniProt Protein Name: Histone deacetylase 11
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC11
• UniProt Synonym Gene Names: HD11

NCBI Description

This gene encodes a class IV histone deacetylase. The encoded protein is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2009]

Uniprot Description

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.