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Western Blot (WB) (Western blot analysis on HeLa cell lysate using RPS9 Antibody, The lane on the left is treated with the antigen-specific peptide.)

Rabbit RPS9 Polyclonal Antibody | anti-RPS9 antibody

RPS9 Antibody

Gene Names
RPS9; S9
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RPS9; Polyclonal Antibody; RPS9 Antibody; 40S ribosomal protein S9; OTTHUMP00000069577; OTTHUMP00000069584; OTTHUMP00000069585; OTTHUMP00000194980; OTTHUMP00000197717; OTTHUMP00000197718; OTTHUMP00000197719; OTTHUMP00000206570; Ribosomal protein S9; rps9; RS9_HUMAN; S9; anti-RPS9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RPS9 antibody detects endogenous levels of total RPS9
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
194
Applicable Applications for anti-RPS9 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:3000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human RPS9
Subcellular Location
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis on HeLa cell lysate using RPS9 Antibody, The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis on HeLa cell lysate using RPS9 Antibody, The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB)

(Western blot analysis on mouse liver lysate using RPS9 Antibody )

Western Blot (WB) (Western blot analysis on mouse liver lysate using RPS9 Antibody )

Immunofluorescence (IF)

(MBS9600311 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunofluorescence (IF) (MBS9600311 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunofluorescence (IF)

(MBS9600311 staining A549  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Immunofluorescence (IF) (MBS9600311 staining A549  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-RPS9 antibody
Description: RPS9 Belongs to the ribosomal protein S4P family. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs.
Subunit Structure: Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.
Similarity: Belongs to the universal ribosomal protein uS4 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 23 kDa
Predicted: 23 kDa
NCBI Official Full Name
40S ribosomal protein S9 isoform a
NCBI Official Synonym Full Names
ribosomal protein S9
NCBI Official Symbol
RPS9
NCBI Official Synonym Symbols
S9
NCBI Protein Information
40S ribosomal protein S9
UniProt Protein Name
40S ribosomal protein S9
Protein Family
UniProt Gene Name
RPS9

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S4P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq, Jul 2008]

Research Articles on RPS9

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Product Notes

The RPS9 rps9 (Catalog #AAA9600311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPS9 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPS9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:3000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the RPS9 rps9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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