Human Inflammasome Antibody Kit

Inflammasome Antibody Sampler Kit

Cat. Num. AAA2549977

• Reactivity: Human
• Applications: Western Blot
• Synonyms: Inflammasome; Inflammasome Antibody Sampler Kit; Inflammasome antibody kit

• Reactivity: Human
• Form/Format: PBS with sodium azide and glycerol.

• Applicable Applications for Inflammasome antibody kit: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Inflammasome Antibody Sampler Kit

Western Blot (WB)

(Western Blot analysis of K562 and hela cell using NLRP3 Polyclonal Antibody at dilution of 1:88)

Western Blot (WB)

(Western Blot analysis of HuvEc cells with AIM2 Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of NIH-3T3 cells using Cleaved-Caspase-1 (D210) Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of 293 cells using Caspase-1 Polyclonal Antibody at dilution of 1:500.)

Western Blot (WB)

(Western Blot analysis of various cells using ASC Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of L929, Mouse brain cells using IL-1beta Polyclonal Antibody at dilution of 1:1000.)

Related Product Information for Inflammasome antibody kit

Product Description
The Inflammasome Antibody Sampler Kit provides an economical means of detecting multiple inflammasome components. The kit contains enough primary antibodies to perform at least two western blot experiments.

Background/Introduction: The innate immune system works as the first line of defense in protection from pathogenic microbes and host-derived signals of cellular distress. One way in which these "danger" signals trigger inflammation is through activation of inflammasomes, which are multiprotein complexes that assemble in the cytosol after exposure to pathogen- associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) and result in the activation of caspase-1 and subsequent cleavage of proinflammatory cytokines IL-1beta and IL-18. Inflammasome complexes typically consist of a cytosolic pattern recognition receptor (PRR; a nucleotide-binding domain and leucine-rich-repeat [NLR] or AIM2-like receptor [ALR] family member), an adaptor protein (ASC/TMS1), and pro-caspase-1. A number of distinct inflammasome complexes have been identified, each with a unique PRR and activation triggers. The best characterized is the NLRP3 complex, which contains NLRP3, ASC, and pro-caspase-1. The NLRP3 inflammasome is activated in a twostep process. First, NF-kappaB signaling is induced through PAMP- or DAMP-mediated activation of TLR4 or TNFR, resulting in increased expression of NLRP3, pro-IL-1beta, and pro-IL-18 (priming step, signal 1). Next, indirect activation of NLRP3 occurs by a multitude of signals (whole pathogens, PAMPs/DAMPs, potassium efflux, lysosomal-damaging environmental factors [uric acid, silica, alum] and endogenous factors [amyloid-beta, cholesterol crystals], and mitochondrial damage), leading to complex assembly and activation of caspase-1 (signal 2). The complex inflammasome structure is built via domain interactions among the protein components. Other inflammasomes are activated by more direct means: double-stranded DNA activates the AIM2 complex, anthrax toxin activates NLRP1, and bacterial flagellin activates NLRC4. Activated caspase-1 induces secretion of proinflammatory cytokines IL-1beta and -18, but also regulates metabolic enzyme expression, phagosome maturation, vasodilation, and pyroptosis, an inflammatory programmed cell death. Inflammasome signaling contributes to the onset of a number of diseases, including atherosclerosis, type II diabetes, Alzheimer's disease, and autoimmune disorders.