Rabbit AIM2 Polyclonal Antibody | anti-AIM2 antibody

AIM2 (N-terminal region) Antibody

Cat. Num. AAA474187

• Gene Names: AIM2; PYHIN4
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Synonyms: AIM2; Polyclonal Antibody; AIM2 (N-terminal region) Antibody; AIM2 (N-terminal region); AIM; anti-AIM2 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using AIM2 (N-terminal region) peptide (without carrier). The antibody detects a 40kDa* doublet corresponding to AIM2 in immunoblots of Jurkat cells, as well as mouse macrophages treated with IFNgamma and LPS.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-AIM2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:1000
• Immunogen: AIM2 synthetic peptide (coupled to KLH) corresponds to amino acid residues in the N-terminal region of human AIM2. This peptide sequence is highly conserved in rat and mouse AIM2.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of human Jurkat cells (lane 1), mouse macrophages untreated (lane 2) and treated (lane 3) with IFNgamma (10 ng/ml) and LPS (1ug/ml) for 12 hr (20 ug/lane). The blot was probed with rabbit polyclonal anti-AIM2 (N-terminal region) antibody at 1:1000.)

Western Blot (WB)

(Western blot analysis of human recombinant AIM2 full length sequence with N-terminal GST tag (62kDa). The blot was probed with rabbit polyclonal anti-AIM2 (N-terminal region) antibody at 1:250 (lane 1) and 1:1000 (lane 2).)

Related Product Information for anti-AIM2 antibody

Host- and pathogen-associated cytoplasmic double-stranded DNA triggers the activation of a NALP3-independent inflammasome, which activates caspase-1, leading to maturation of pro-interleukin-1beta and inflammation. Several studies have isolated AIM2 (absent in melanoma 2) as a candidate cytoplasmic-DNA-sensing protein that contains an N-terminal pyrin domain and C-terminal oligonucleotide binding domain. A screen for transcripts induced by interferon-beta identified AIM2 gene expression. AIM2 protein bound double-stranded DNA, recruited the inflammasome adaptor ASC, and localized to ASC containing speckles. AIM2 and ASC form a pyroptosome, which induces pyroptotic cell death mediated by caspase-1. RNA-mediated suppression of AIM2 expression impairs DNA-induced maturation of interleukin-1beta in THP-1 human monocytic cells, as well as abrogates caspase-1 activation in response to cytoplasmic double-stranded DNA and the double-stranded DNA vaccinia virus. Thus, AIM2 is a DNA-sensing protein for the activation of the caspase-1 inflammasome.

References

Bürckstümmer, T. et al. (2009). Nat Immunol. 10(3):266.
Fernandes-Alnemri, T. (2009) Nature. 458(7237):509.
Hornung, V. et al. (2009). Nature. 458(7237):514.
Roberts, T.L. et al. (2009). Science. 323(5917):1057.

NCBI and Uniprot Product Information

• NCBI GI #: 4757734
• NCBI GeneID: 9447
• NCBI Accession #: NP_004824.1
• NCBI GenBank Nucleotide #: NM_004833.1
• UniProt Accession #: O14862; Q5T3V9; Q96FG9; A8K7M7
• Molecular Weight: 38,954 Da
• NCBI Official Full Name: interferon-inducible protein AIM2
• NCBI Official Synonym Full Names: absent in melanoma 2
• NCBI Official Symbol: AIM2
• NCBI Official Synonym Symbols: PYHIN4
• NCBI Protein Information: interferon-inducible protein AIM2
• UniProt Protein Name: Interferon-inducible protein AIM2
• Protein Family: Protein
• UniProt Gene Name: AIM2
• UniProt Entry Name: AIM2_HUMAN

NCBI Description

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq, Jul 2008]

Uniprot Description

AIM2: a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. Induced by interferon gamma. Defects in AIM2 may be a cause of microsatellite unstable colon cancers. AIM2 and various NOD-like receptors form multiprotein complexes called inflammasomes, which mediate caspase-1-dependent processing of pro-IL-1beta.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; double-stranded DNA binding

Biological Process: positive regulation of protein oligomerization; positive regulation of interleukin-1 beta production; tumor necrosis factor-mediated signaling pathway; apoptosis; inhibition of NF-kappaB transcription factor; innate immune response; immune response; interleukin-1 beta secretion; positive regulation of interleukin-1 beta secretion; inflammatory response; positive regulation of defense response to virus by host; activation of innate immune response; activation of NF-kappaB transcription factor