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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Cattle Signal Transducer And Activator Of Transcription 3 (STAT3) Polyclonal Antibody | anti-STAT3 antibody

Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)

Reactivity
Cattle
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Signal Transducer And Activator Of Transcription 3 (STAT3); Polyclonal Antibody; Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3); anti-STAT3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Cattle
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against STAT3. It has been selected for its ability to recognize STAT3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-VWLDNIID LVKKYILALW NEGYIMGFIS KERERAILST KPPGTFLLRF SESSKEGGVT FTWVEKDISG KTQIQSVEPY TKQQLNNMSF AEIIMGYKIM DATNILVSPL
Sequence Length
770
Applicable Applications for anti-STAT3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant STAT3 (Val563~Leu670) expressed in E.coli.
Cross Reactivity
Bovine
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2058518
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
87,975 Da
NCBI Official Full Name
signal transducer and activator of transcription 3
NCBI Official Synonym Full Names
signal transducer and activator of transcription 3
NCBI Official Symbol
STAT3
NCBI Protein Information
signal transducer and activator of transcription 3
UniProt Protein Name
Signal transducer and activator of transcription 3
UniProt Gene Name
STAT3

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription factor

Cellular Component: cytoplasm; nucleus; plasma membrane

Molecular Function: chromatin DNA binding; DNA binding; DNA binding transcription factor activity; protein dimerization activity; protein homodimerization activity; protein kinase binding; signal transducer activity

Biological Process: astrocyte differentiation; eating behavior; eye photoreceptor cell differentiation; glucose homeostasis; inflammatory response; leptin-mediated signaling pathway; phosphorylation; positive regulation of erythrocyte differentiation; positive regulation of Notch signaling pathway; positive regulation of transcription, DNA-templated; protein import into nucleus; radial glial cell differentiation; regulation of cell cycle; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; sexual reproduction; thermoregulation; transcription, DNA-dependent

Research Articles on STAT3

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Product Notes

The STAT3 stat3 (Catalog #AAA2005676) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3) reacts with Cattle and may cross-react with other species as described in the data sheet. AAA Biotech's Signal Transducer And Activator Of Transcription 3 (STAT3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the STAT3 stat3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-VWLDNI ID LVKKYILALW NEGYIMGFIS KERERAILST KPPGTFLLRF SESSKEGGVT FTWVEKDISG KTQIQSVEPY TKQQLNNMSF AEIIMGYKIM DATNILVSPL. It is sometimes possible for the material contained within the vial of "Signal Transducer And Activator Of Transcription 3 (STAT3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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