Human Histone Deacetylase (HDAC) Antibody Kit | HDAC antibody kit

Histone Deacetylase (HDAC) Antibody Sampler Kit

Cat. Num. AAA2550041

• Reactivity: Human
• Applications: Western Blot
• Synonyms: Histone Deacetylase (HDAC); Histone Deacetylase (HDAC) Antibody Sampler Kit; HDAC antibody kit

• Reactivity: Human
• Form/Format: PBS with sodium azide and glycerol.

• Applicable Applications for HDAC antibody kit: Western Blot (WB)
• Application Notes: Western Blot (WB): 1:500-1:2000
• Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of 293 cells using HDAC1 Polyclonal Antibody at dilution of 1:500.)

Western Blot (WB)

(Western Blot analysis of Jurkat, 3T3 cells using HDAC2 Polyclonal Antibody at dilution of 1:2000.)

Western Blot (WB)

(Western Blot analysis of 3T3 cells with Phospho-HDAC2 (Ser394) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of Hela cells using HDAC3 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of Hela cells using HDAC4 Polyclonal Antibody at dilution of 1:2000.)

Western Blot (WB)

(Western Blot analysis of 293 cells with Phospho-HDAC4 (Ser632) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of K562, 3T3 cells with HDAC5 Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of VEC cells with Phospho-HDAC5 (Ser498) Polyclonal Antibody at dilution of 1:500)

Western Blot (WB)

(Western Blot analysis of 3T3, Rat intestine cells using HDAC5/9 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of HepG2 cells using HDAC6 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of 3T3 cells with Phospho-HDAC6 (Ser22) Polyclonal Antibody at dilution of 1:500)

Related Product Information for HDAC antibody kit

Background:
Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription. HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes. In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity. Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases. Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents.
Description:
The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.