Human IL-32 (Interleukin 32) ELISA Kit | IL-32 elisa kit

Human IL-32 (Interleukin 32) Pre-Coated ELISA Kit

Cat. Num. AAA669194

• Gene Names: IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
• Reactivity: Human
• Applications: ELISA
• Synonyms: IL-32 (Interleukin 32); Human IL-32 (Interleukin 32) Pre-Coated ELISA Kit; IL-32 elisa kit

• Reactivity: Human
• Sequence Length: 234

• Applicable Applications for IL-32 elisa kit: ELISA (EIA)

Typical Testing Data/Standard Curve (for reference only)

(Fig-1: Human IL-32 Standard Curve)

Related Product Information for IL-32 elisa kit

Background/Introduction: The innate immune system works as the first line of defense in protection from pathogenic microbes and host-derived signals of cellular distress. One way in which these "danger" signals trigger inflammation is through activation of inflammasomes, which are multiprotein complexes that assemble in the cytosol after exposure to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) and result in the activation of caspase-1 and subsequent cleavage of proinflammatory cytokines IL-1beta and IL-18. Inflammasome complexes typically consist of a cytosolic pattern recognition receptor (PRR; a nucleotide-binding domain and leucine-rich-repeat [NLR] or AIM2-like receptor [ALR] family member), an adaptor protein (ASC/TMS1), and pro-caspase-1. A number of distinct inflammasome complexes have been identified, each with a unique PRR and activation triggers. The best characterized is the NLRP3 complex, which contains NLRP3, ASC, and pro-caspase-1. The NLRP3 inflammasome is activated in a twostep process. First, NF-kappaB signaling is induced through PAMP-or DAMP-mediated activation of TLR4 or TNFR, resulting in increased expression of NLRP3, pro-IL-1beta, and pro-IL-18 (priming step, signal 1). Next, indirect activation of NLRP3 occurs by a multitude of signals (whole pathogens, PAMPs/DAMPs, potassium efflux, lysosomal-damaging environmental factors [uric acid, silica, alum] and endogenous factors [amyloid-beta, cholesterol crystals], and mitochondrial damage), leading to complex assembly and activation of caspase-1 (signal 2). The complex inflammasome structure is built via domain interactions among the protein components. Other inflammasomes are activated by more direct means: double-stranded DNA activates the AIM2 complex, anthrax toxin activates NLRP1, and bacterial flagellin activates NLRC4. Activated caspase-1 induces secretion of proinflammatory cytokines IL-1beta and-18, but also regulates metabolic enzyme expression, phagosome maturation, vasodilation, and pyroptosis, an inflammatory programmed cell death. Inflammasome signaling contributes to the onset of a number of diseases, including atherosclerosis, type II diabetes, Alzheimer's disease, and autoimmune disorders.

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NCBI and Uniprot Product Information

• NCBI GI #: 815890841
• NCBI GeneID: 9235
• NCBI Accession #: NP_001295007.1
• NCBI GenBank Nucleotide #: NM_001308078.1
• UniProt Accession #: P24001; Q5VFH7; Q5VFH8; Q8WV38; Q96GK9; A6NNM0; A8MPX0; B4DJM1; B8Q191; D3DUB0; D3DUB2
• Molecular Weight: 17,003 Da
• NCBI Official Full Name: interleukin-32 isoform E
• NCBI Official Synonym Full Names: interleukin 32
• NCBI Official Symbol: IL32
• NCBI Official Synonym Symbols: NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
• NCBI Protein Information: interleukin-32
• UniProt Protein Name: Interleukin-32
• UniProt Gene Name: IL32
• UniProt Synonym Gene Names: NK4; TAIF; IL-32

NCBI Description

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.