Testing Data
Nigericin Antibiotic
Nigericin
Synonyms
Nigericin; Nigericin antibiotic
Sequence Length
145
Application Notes
1. Reconstitute each vial of Nigericin with 100 uL DMSO to form the 5 mM stock concentrate. Once reconstituted, it may be aliquoted and stored at <= -20 degree C for 1 year protected from light and thawed no more than twice during that time.
2. Immediately prior to addition to the samples and controls, dilute 5 mM Nigericin stock 1:10 in diH2O to form a 500 uM working solution for use in treating samples. For example, dilute 1:10 by adding 20 uL stock concentrate to 180 uL diH2O.
3. Use Nigericin at 1-20 uM to induce NLRP3 inflammasome activation in cells. For example, to use at 10 uM, dilute 500 uM working solution 1:50 in samples; e.g., spike 294 uL cell suspension/overlay medium with 6 uL of 500 uM working solution. Typical treatment periods range from 3-24 hours at 37 degree C. Each investigator should adjust the concentration of Nigericin and treatment period to accommodate the particular cell line and research conditions.
4. NLRP3 inflammasome activation can be detected by ELISA or Western blot measuring secreted pro-inflammatory cytokines IL-1beta or IL-18, or through the use of caspase-1 activation assays, such as ICT's Caspase-1 Assay Kits or Pyroptosis/Caspase-1 Assay Kit.
2. Immediately prior to addition to the samples and controls, dilute 5 mM Nigericin stock 1:10 in diH2O to form a 500 uM working solution for use in treating samples. For example, dilute 1:10 by adding 20 uL stock concentrate to 180 uL diH2O.
3. Use Nigericin at 1-20 uM to induce NLRP3 inflammasome activation in cells. For example, to use at 10 uM, dilute 500 uM working solution 1:50 in samples; e.g., spike 294 uL cell suspension/overlay medium with 6 uL of 500 uM working solution. Typical treatment periods range from 3-24 hours at 37 degree C. Each investigator should adjust the concentration of Nigericin and treatment period to accommodate the particular cell line and research conditions.
4. NLRP3 inflammasome activation can be detected by ELISA or Western blot measuring secreted pro-inflammatory cytokines IL-1beta or IL-18, or through the use of caspase-1 activation assays, such as ICT's Caspase-1 Assay Kits or Pyroptosis/Caspase-1 Assay Kit.
Preparation and Storage
Store at < = -20 degree C.
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Nigericin antibiotic
Description: Nigericin is a potent microbial toxin that acts as a potassium ionophore, inducing a net decrease in intracellular levels of potassium. This is critical for the oligomerization of the NLRP3 inflammasome and activation of caspase-1 in pyroptosis. Nigericin can be used as a positive control in pyroptosis experiments.
Background: Inflammatory caspases, such as caspase-1, play a critical role in the mechanism to trigger the lytic programmed cell death known as pyroptosis. Nigericin can be utilized as a positive control in pyroptosis experiments.
Nigericin is a potent microbial toxin derived from Streptomyces hygroscopicus. It acts as a potassium ionophore, inducing a net decrease in intracellular levels of potassium, which is crucial for oligomerization of the NLRP3 inflammasome and activation of caspase-1. It requires signaling through pannexin-1 to induce caspase-1 activation and IL-1beta processing and release. It has been shown to generate a robust caspase-1 activation response in various cell lines, including Jurkat and THP-I cells.
Background: Inflammatory caspases, such as caspase-1, play a critical role in the mechanism to trigger the lytic programmed cell death known as pyroptosis. Nigericin can be utilized as a positive control in pyroptosis experiments.
Nigericin is a potent microbial toxin derived from Streptomyces hygroscopicus. It acts as a potassium ionophore, inducing a net decrease in intracellular levels of potassium, which is crucial for oligomerization of the NLRP3 inflammasome and activation of caspase-1. It requires signaling through pannexin-1 to induce caspase-1 activation and IL-1beta processing and release. It has been shown to generate a robust caspase-1 activation response in various cell lines, including Jurkat and THP-I cells.
Product Categories/Family for Nigericin antibiotic
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
746.94g/mol
NCBI Official Full Name
nigBI epoxide hydrolase involved in nigericin biosynthesis
Similar Products
Product Notes
The Nigericin (Catalog #AAA258365) is an Antibiotic and is intended for research purposes only. The product is available for immediate purchase. 1. Reconstitute each vial of Nigericin with 100 uL DMSO to form the 5 mM stock concentrate. Once reconstituted, it may be aliquoted and stored at <= -20 degree C for 1 year protected from light and thawed no more than twice during that time. 2. Immediately prior to addition to the samples and controls, dilute 5 mM Nigericin stock 1:10 in diH2O to form a 500 uM working solution for use in treating samples. For example, dilute 1:10 by adding 20 uL stock concentrate to 180 uL diH2O. 3. Use Nigericin at 1-20 uM to induce NLRP3 inflammasome activation in cells. For example, to use at 10 uM, dilute 500 uM working solution 1:50 in samples; e.g., spike 294 uL cell suspension/overlay medium with 6 uL of 500 uM working solution. Typical treatment periods range from 3-24 hours at 37 degree C. Each investigator should adjust the concentration of Nigericin and treatment period to accommodate the particular cell line and research conditions. 4. NLRP3 inflammasome activation can be detected by ELISA or Western blot measuring secreted pro-inflammatory cytokines IL-1beta or IL-18, or through the use of caspase-1 activation assays, such as ICT's Caspase-1 Assay Kits or Pyroptosis/Caspase-1 Assay Kit. Researchers should empirically determine the suitability of the Nigericin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nigericin, Antibiotic" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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