Mouse Chordin PicoKine ELISA Kit | Chrd elisa kit

Mouse Chordin PicoKine ELISA Kit

Cat. Num. AAA177587

• Gene Names: Chrd; Chd
• Reactivity: Mouse
• Synonyms: Chordin PicoKine; Mouse Chordin PicoKine ELISA Kit; Chordin; Chrd; CHRD_HUMAN; MGC133038; Shortgastrulation protein; SOG ; chordin; Chrd elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Chordin
• Sequence Length: 970

• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of Mouse Chordin in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, T27-S948
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse Chordin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Chordin standard solution and each sample is measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|98|319|1248|75|402|1866; Standard deviation|5.19|18.82|82.36|4.35|28.94|136.21; CV(%)|5.3|5.9|6.6|5.8|7.2|7.3
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.005|0.074|0.144|0.267|0.556|0.966|1.492|1.992
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse Chordin PicoKine ELISA Kit standard curve )

Related Product Information for Chrd elisa kit

Principle of the assay: Mouse Chordin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Chordin has been precoated onto 96-well plates. Standards (NSO, T27-S948) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chordin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Chordin amount of sample captured in plate.

Background: Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms. Chordin is also involved in avian gastrulation. It is expressed in the anterior cells of Koller's sickle, which form the anterior cells of the primitive streak, a key structure through which gastrulation occurs.

NCBI and Uniprot Product Information

• NCBI GI #: 487442580
• NCBI GeneID: 12667
• NCBI Accession #: NP_001264970.1
• NCBI GenBank Nucleotide #: NM_001278041.1
• UniProt Accession #: Q9Z0E2
• Molecular Weight: 101,513 Da
• NCBI Official Full Name: chordin isoform 2
• NCBI Official Synonym Full Names: chordin
• NCBI Official Symbol: Chrd
• NCBI Official Synonym Symbols: Chd
• NCBI Protein Information: chordin
• UniProt Protein Name: Chordin
• Protein Family: Chordin
• UniProt Gene Name: Chrd
• UniProt Entry Name: CHRD_MOUSE

Uniprot Description

CHRD: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. Belongs to the chordin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Cell adhesion; Secreted, signal peptide

Cellular Component: extracellular region; extracellular space

Molecular Function: heparin binding; protein binding; syndecan binding

Biological Process: BMP signaling pathway in spinal cord dorsal-ventral patterning; central nervous system development; dorsal/ventral pattern formation; forebrain development; gastrulation with mouth forming second; mesoderm formation; multicellular organismal development; negative regulation of BMP signaling pathway; negative regulation of cell migration; negative regulation of osteoblast differentiation; osteoblast differentiation; pattern specification process; positive regulation of cell adhesion; positive regulation of mesenchymal cell proliferation; skeletal development