Western Blot (WB)
(Anti- NR3C1 antibody, MBS177524, Western blottingAll lanes: Anti NR3C1 (MBS177524) at 0.5ug/mlWB: Recombinant Human NR3C1 Protein 0.5ngPredicted bind size: 38KDObserved bind size: 38KD)
Rabbit Glucocorticoid receptor Polyclonal Antibody | anti-NR3C1 antibody
Anti-NR3C1 Antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Rat
Western Blot (WB)
(Anti- NR3C1 antibody, MBS177524, Western blottingAll lanes: Anti NR3C1 (MBS177524) at 0.5ug/mlWB: Recombinant Human NR3C1 Protein 0.5ngPredicted bind size: 38KDObserved bind size: 38KD)
Western Blot (WB)
(Anti- NR3C1 antibody, MBS177524, Western blottingAll lanes: Anti NR3C1 (MBS177524) at 0.5ug/mlWB: Recombinant Human NR3C1 Protein 0.5ngPredicted bind size: 38KDObserved bind size: 38KD)
Western Blot (WB)
(Anti- NR3C1 antibody, MBS177524, Western blottingAll lanes: Anti NR3C1 (MBS177524) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: MM231 Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 90KDObserved bind size: 90KD )
Western Blot (WB)
(Anti- NR3C1 antibody, MBS177524, Western blottingAll lanes: Anti NR3C1 (MBS177524) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: MM231 Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 90KDObserved bind size: 90KD )
Immunohistochemistry (IHC)
(Anti- NR3C1 antibody, MBS177524,IHC(P)IHC(P): Rat Cardiac Muscle Tissue )
Immunohistochemistry (IHC)
(Anti- NR3C1 antibody, MBS177524,IHC(P)IHC(P): Rat Cardiac Muscle Tissue )
Immunohistochemistry (IHC)
(Anti- NR3C1 antibody, MBS177524,IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti- NR3C1 antibody, MBS177524,IHC(P)IHC(P): Human Mammary Cancer Tissue )
Background: The glucocorticoid receptor (GR, or GCR), also known as NR3C1, is the receptor to which cortisol and other glucocorticoids bind. In humans, the GR protein is encoded by NR3C1 gene which is located on chromosome 5 (5q31). GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. Because the receptor gene is expressed in several forms, it has many different (pleiotropic) effects in different parts of the body. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol (by preventing the translocation of other transcription factors from the cytosol into the nucleus).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]
Uniprot Description
GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described.
Protein type: Mitochondrial; Nuclear receptor; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 5q31.3
Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; cytosol; nucleus
Molecular Function: protein dimerization activity; protein binding; glucocorticoid receptor activity; zinc ion binding; transcription factor activity; steroid binding
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; adrenal gland development; maternal behavior; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid metabolic process; glucocorticoid mediated signaling; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; glucocorticoid receptor signaling pathway; regulation of gluconeogenesis; gene expression; positive regulation of transcription from RNA polymerase II promoter
Disease: Glucocorticoid Resistance, Generalized
Research Articles on NR3C1
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Product Notes
The NR3C1 nr3c1 (Catalog #AAA177524) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NR3C1 Antibody reacts with Human, Rat. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Glucocorticoid receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC-P). Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Rat. Researchers should empirically determine the suitability of the NR3C1 nr3c1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucocorticoid receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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