Mouse CD86/B7-2 PicoKine ELISA Kit | Cd86 elisa kit

Mouse CD86/B7-2 PicoKine ELISA Kit

Cat. Num. AAA177620

• Gene Names: Cd86; B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
• Reactivity: Mouse
• Synonyms: CD86/B7-2 PicoKine; Mouse CD86/B7-2 PicoKine ELISA Kit; T-lymphocyte activation antigen CD86; Activation B7 2 antigen; B7 2; B70; B72; BU63; CD28LG2; CD86 antigen (CD28 antigen ligand 2 B7 2 antigen); CD86 molecule; CTLA 4 counter receptor B7.2; FUN 1; FUN1; T lymphocyte activation antigen CD86 precursor; Cd86 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CD86
• Sequence Length: 309

• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of mouse CD86 in cell culture supernates and serum.
• Immunogen: NSO, V26-G245
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse CD86 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse CD86 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(ng/ml)|0.21|1.44|2.59|0.23|1.47|2.46; Standard deviation|0.011|0.084|0.161|0.013|0.095|0.150; CV(%)|5.3|5.9|6.2|5.8|6.5|6.1
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.067|0.125|0.170|0.307|0.525|0.897|1.449|2.211
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse CD86/B7-2 PicoKine ELISA Kit standard curve )

Related Product Information for Cd86 elisa kit

Principle of the assay: Mouse CD86 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD86 has been precoated onto 96-well plates. Standards (NSO, V26-G245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD86 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD86 amount of sample captured in plate.

Background: Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

NCBI and Uniprot Product Information

• NCBI GI #: 161484599
• NCBI GeneID: 12524
• NCBI Accession #: NP_062261.3
• NCBI GenBank Nucleotide #: NM_019388.3
• UniProt Accession #: P42082
• Molecular Weight: 33,962 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD86
• NCBI Official Synonym Full Names: CD86 antigen
• NCBI Official Symbol: Cd86
• NCBI Official Synonym Symbols: B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
• NCBI Protein Information: T-lymphocyte activation antigen CD86
• UniProt Protein Name: T-lymphocyte activation antigen CD86
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: Cd86
• UniProt Synonym Gene Names: ETC-1
• UniProt Entry Name: CD86_MOUSE

Uniprot Description

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Cellular Component: cell surface; external side of plasma membrane; integral to membrane; intracellular membrane-bound organelle; membrane; plasma membrane

Molecular Function: receptor binding

Biological Process: adaptive immune response; B cell activation; defense response to virus; immune system process; negative regulation of T cell anergy; positive regulation of activated T cell proliferation; positive regulation of T cell proliferation; toll-like receptor 3 signaling pathway; toll-like receptor signaling pathway