Typical Testing Data/Standard Curve (for reference only)
(Rat GDF-15 PicoKine ELISA Kit standard curve )
Rat GDF-15 ELISA Kit | GDF15 elisa kit
Rat GDF-15 PicoKine ELISA Kit
Reactivity
Rat
Synonyms
GDF-15; Rat GDF-15 PicoKine ELISA Kit; gdf 15; GDF15; MIC 1; MIC1; MIC-1; NAG-1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; PLAB; PTGFB; Growth/differentiation factor 15; GDF15 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat GDF-15
Sequence Length
303
Assay Range
15.6pg/ml-1000pg/ml
Sensitivity
<10pg/ml
Anticoagulant
heparin or EDTA
TMB incubation time
15-20
Immunogen
NSO, S189-A303
Sample Data
Concentration(pg/ml): |0|15.6|31.2|62.5|125|250|500|1000
O.D.: |0.027|0.141|0.272|0.477|0.899|1.591|2.073|2.299
O.D.: |0.027|0.141|0.272|0.477|0.899|1.591|2.073|2.299
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n: |16|16|16|24|24|24
Mean(pg/ml): |43.7|139|601|52.3|154|714
Standard Deviation: |1.88|6.81|33.06|3.09|10.16|45.70
CV(%): |4.3|4.9|5.5|5.9|6.6|6.4
n: |16|16|16|24|24|24
Mean(pg/ml): |43.7|139|601|52.3|154|714
Standard Deviation: |1.88|6.81|33.06|3.09|10.16|45.70
CV(%): |4.3|4.9|5.5|5.9|6.6|6.4
Assay Procedure Step 1
Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml rat GDF-15 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each rat GDF-15 standard solution and each sample be measured in duplicate.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
(Rat GDF-15 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat GDF-15 PicoKine ELISA Kit standard curve )
Related Product Information for GDF15 elisa kit
For quantitative detection of rat GDF-15 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: GDF-15(Growth differentiation factor 15), also known as TGF-PL, MIC-1, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.
Principle: Rat GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for GDF-15 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: S189-A303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat GDF-15 amount of sample captured in plate.
Background: GDF-15(Growth differentiation factor 15), also known as TGF-PL, MIC-1, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.
Principle: Rat GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for GDF-15 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: S189-A303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat GDF-15 amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,439 Da
NCBI Official Full Name
growth/differentiation factor 15
NCBI Official Synonym Full Names
growth differentiation factor 15
NCBI Official Symbol
Gdf15
NCBI Protein Information
growth/differentiation factor 15
UniProt Protein Name
Growth/differentiation factor 15
Protein Family
UniProt Gene Name
Gdf15
UniProt Synonym Gene Names
Sbf; GDF-15
NCBI Description
member of the TGF-beta superfamily of growth factors [RGD, Feb 2006]
Research Articles on GDF15
Similar Products
Product Notes
The Rat GDF15 gdf15 (Catalog #AAA178748) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178748 ELISA Kit recognizes Rat GDF15. It is sometimes possible for the material contained within the vial of "GDF-15, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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