Typical Testing Data/Standard Curve
Lactose Assay Kit | LAC assay kit
Lactose Assay Kit
Synonyms
Lactose; Lactose Assay Kit; LAC assay kit
Sequence Length
65
Preparation and Storage
Store the 10X Assay Buffer at room temperature
Store all other components at -20 degree C
The Fluorometric Probe is light sensitive and must be protected accordingly
Avoid multiple freeze/thaw cycles.
Store all other components at -20 degree C
The Fluorometric Probe is light sensitive and must be protected accordingly
Avoid multiple freeze/thaw cycles.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for LAC assay kit
Principle of the Assay||Lactose Assay Kit measures total lactose within food or biological samples. First, lactase cleaves lactose into glucose and galactose. Glucose is oxidized by glucose oxidase into D-gluconic acid plus hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of lactose standard within the 96-well microtiter plate format. Samples and standards are incubated for 45 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).
Background/Introduction: Lactose is a disaccharide form of sugar made up of one glucose and one galactose monosaccharide. In animals, millimolar levels of lactose are found in breast milk. In order for lactose to be absorbed into the bloodstream, lactase (secreted from the small intestines) cleaves lactose into glucose and galactose monosaccharides. The inability to digest lactose is known as lactose intolerance, which arises from a reduction or loss in production of lactase, and is now more commonly known as "lactase deficiency". In most mammals, lactase deficiency is a normal condition that occurs shortly after weaning from breast milk as the primary food source. In humans, a significant population remains tolerant to lactose into adulthood due to the continued production of lactase in the small intestines. Some estimates suggest that 75% of the world's adult population is lactase deficient. Since the severity of lactose maldigestion symptoms can depend on the amount of lactose consumed, it is important to quantify the relative amounts of lactose in various dairy food sources. Lactose Assay Kit is a simple fluorometric assay that measures the amount of total lactose present in milk based food products or biological samples (such as blood or urine from lactating animals) in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, lactose standards, endogenous controls*, and unknown samples. Sample lactose concentrations are determined by comparison with a known lactose standard. The kit has a detection sensitivity limit of 10 uM lactose.
Background/Introduction: Lactose is a disaccharide form of sugar made up of one glucose and one galactose monosaccharide. In animals, millimolar levels of lactose are found in breast milk. In order for lactose to be absorbed into the bloodstream, lactase (secreted from the small intestines) cleaves lactose into glucose and galactose monosaccharides. The inability to digest lactose is known as lactose intolerance, which arises from a reduction or loss in production of lactase, and is now more commonly known as "lactase deficiency". In most mammals, lactase deficiency is a normal condition that occurs shortly after weaning from breast milk as the primary food source. In humans, a significant population remains tolerant to lactose into adulthood due to the continued production of lactase in the small intestines. Some estimates suggest that 75% of the world's adult population is lactase deficient. Since the severity of lactose maldigestion symptoms can depend on the amount of lactose consumed, it is important to quantify the relative amounts of lactose in various dairy food sources. Lactose Assay Kit is a simple fluorometric assay that measures the amount of total lactose present in milk based food products or biological samples (such as blood or urine from lactating animals) in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, lactose standards, endogenous controls*, and unknown samples. Sample lactose concentrations are determined by comparison with a known lactose standard. The kit has a detection sensitivity limit of 10 uM lactose.
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Product Notes
The Lactose lac (Catalog #AAA169244) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Lactose, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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