Activity
(Activity: Sample activity data. For lot-specific data, please refer to lot-specific datasheet.)
USP1 recombinant protein
USP1, Active
Synonyms
USP1; Active; Ubiquitin carboxyl-terminal hydrolase 1; USP1 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.25mM DTT, 0.1mM EDTA, 25% glycerol.
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles
Activity
(Activity: Sample activity data. For lot-specific data, please refer to lot-specific datasheet.)
Activity
(Activity: Sample activity data. For lot-specific data, please refer to lot-specific datasheet.)
SDS-Page
(Purity: Sample purity data. For lot-specific data, please refer to lot-specific datasheets.)
SDS-Page
(Purity: Sample purity data. For lot-specific data, please refer to lot-specific datasheets.)
Related Product Information for USP1 recombinant protein
Recombinant human full-length USP1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_003368.
Ubiquitin Specific Protease 1 (USP1) is a human deubiquitinase (DUB) that contains one of the largest catalytic domains within the USP family. USP1 plays a role in regulating cellular response to DNA damage and is also involved in the control of cell differentiation (1). USP1 regulates ubiquitination of Proliferating Cell Nuclear Antigen (PCNA) in response to DNA damage preventing unscheduled recruitment of error-prone TLS DNA polymerases [2]. USP1 expression is deregulated in certain types of human cancer and is thus emerging as a novel target in cancer therapy (3).
Ubiquitin Specific Protease 1 (USP1) is a human deubiquitinase (DUB) that contains one of the largest catalytic domains within the USP family. USP1 plays a role in regulating cellular response to DNA damage and is also involved in the control of cell differentiation (1). USP1 regulates ubiquitination of Proliferating Cell Nuclear Antigen (PCNA) in response to DNA damage preventing unscheduled recruitment of error-prone TLS DNA polymerases [2]. USP1 expression is deregulated in certain types of human cancer and is thus emerging as a novel target in cancer therapy (3).
Product Categories/Family for USP1 recombinant protein
References
1. Olazabal-Herrero, A. et al: Structure-function analysis of USP1: Insights into the role of Ser313 phosphorylation site and the effect of cancer-associated mutations on autocleavage. Mol Cancer. 14(1):33, 2015.
2. Huang, T.T. et al: Regulation of monoubiquitinated PCNA by DUB autocleavage. Nat Cell Biol. 8:339-47, 2006.
3. García-Santisteban, I. et al: USP1 deubiquitinase: Cellular functions, regulatory mechanisms and emerging potential as target in cancer therapy. Mol Cancer 12:91, 2013.
2. Huang, T.T. et al: Regulation of monoubiquitinated PCNA by DUB autocleavage. Nat Cell Biol. 8:339-47, 2006.
3. García-Santisteban, I. et al: USP1 deubiquitinase: Cellular functions, regulatory mechanisms and emerging potential as target in cancer therapy. Mol Cancer 12:91, 2013.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
approx. MW: 130kDa
Protein Family
Similar Products
Product Notes
The USP1 (Catalog #AAA517757) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "USP1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
