Activity
(Specific Activity : Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
NEK10 recombinant protein
NEK10, Active
Synonyms
NEK10; Active; NIMA (Never In Mitosis Gene A) Related Kinase 10; NEK10 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity : Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity : Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical datasheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical datasheet.)
Related Product Information for NEK10 recombinant protein
Recombinant human NEK10 (266-895) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_152534.
NEK10 (NIMA Related Kinase 10) is a serine/threonine kinase that is involved in the regulation of the G2/M checkpoint in mitosis. It has unique structure with central catalytic kinase domain flanked by two large regulatory domains (1). NEK10 acts as a positive regulator of ERK1/2 in response to UV irradiation. In mitochondria, NEK10 interacts with GLUD1 and citrate synthase and plays a role in maintaining mitochondrial homeostasis (2). NEK10 also contributes to cilium formation through interaction with PKA and PCM1 and participating in a cAMP dependent pathway [3]. Mutations in NEK10 have been reported in lung cancer and breast cancer (2).
NEK10 (NIMA Related Kinase 10) is a serine/threonine kinase that is involved in the regulation of the G2/M checkpoint in mitosis. It has unique structure with central catalytic kinase domain flanked by two large regulatory domains (1). NEK10 acts as a positive regulator of ERK1/2 in response to UV irradiation. In mitochondria, NEK10 interacts with GLUD1 and citrate synthase and plays a role in maintaining mitochondrial homeostasis (2). NEK10 also contributes to cilium formation through interaction with PKA and PCM1 and participating in a cAMP dependent pathway [3]. Mutations in NEK10 have been reported in lung cancer and breast cancer (2).
Product Categories/Family for NEK10 recombinant protein
References
1. Meirelles, G.V. et al: Stop Ne(c)king around: How interactomics contributes to functionally characterize Nek family kinases. World J Biol Chem. 5(2):141-60, 2014.
2. Peres de Oliveira, A. et al: NEK10 interactome and depletion reveal new roles in mitochondria. Proteome Sci. 18:4, 2020.
3. Porpora, M. et al: Counterregulation of cAMP-directed kinase activities controls ciliogenesis. Nat Commun. 9(1):1224, 2018.
2. Peres de Oliveira, A. et al: NEK10 interactome and depletion reveal new roles in mitochondria. Proteome Sci. 18:4, 2020.
3. Porpora, M. et al: Counterregulation of cAMP-directed kinase activities controls ciliogenesis. Nat Commun. 9(1):1224, 2018.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
approx. MW: 98kDa
Protein Family
Similar Products
Product Notes
The NEK10 (Catalog #AAA517312) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NEK10, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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