Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
CDK9/CyclinT1 Recombinant Protein | CDK9 recombinant protein
CDK9/CyclinT1, Active
Synonyms
CDK9/CyclinT1; Active; CDK9: CDC2L4; C-2k; TAK; PITALRE CyclinT1: CCNT; CYCT1; HIVE1; CDK9 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for CDK9 recombinant protein
Recombinant full-length human CDK9 and CyclinT1 proteins were co-expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. The gene accession numbers for CDK9 and CyclinT1 are NM_001261 and, NM_001240, respectively.
Cyclin-dependent kinase 9 (Cdk9) is a cdc2-like serine/threonine kinase that associates with cyclin T1 to form a heterodimer. CDK9/Cyclin T1 heterodimer is the main component of the positive transcription elongation factor (P-TEFb) which is critical for the stabilization of the elongation process of RNA polymerase II generated transcripts (1). P-TEFb containing Cdk9 and cyclin T1 is essential for the replication of the human immunodeficiency virus type 1 and type 2 in human cells (2). Cdk9-associated pathway are linked to human malignancies and cardiac hypertrophy (3).
Cyclin-dependent kinase 9 (Cdk9) is a cdc2-like serine/threonine kinase that associates with cyclin T1 to form a heterodimer. CDK9/Cyclin T1 heterodimer is the main component of the positive transcription elongation factor (P-TEFb) which is critical for the stabilization of the elongation process of RNA polymerase II generated transcripts (1). P-TEFb containing Cdk9 and cyclin T1 is essential for the replication of the human immunodeficiency virus type 1 and type 2 in human cells (2). Cdk9-associated pathway are linked to human malignancies and cardiac hypertrophy (3).
Product Categories/Family for CDK9 recombinant protein
References
1. Gaetano, R. et. al: Role of the cyclin-dependent kinase 9-related pathway in mammalian gene expression and human diseases. Cell Cycle. 7:23, 3664-3668, 2008.
2. Lin, X. et. al: P-TEFb containing cyclin K and Cdk9 can activate transcription via RNA. J Biol Chem. 277:16873-8, 2002.
3. Malumbres, M. et. al: CDK inhibitors in cancer therapy: what is next? Trends Pharmacol Sci. 29:16-21, 2008.
2. Lin, X. et. al: P-TEFb containing cyclin K and Cdk9 can activate transcription via RNA. J Biol Chem. 277:16873-8, 2002.
3. Malumbres, M. et. al: CDK inhibitors in cancer therapy: what is next? Trends Pharmacol Sci. 29:16-21, 2008.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
approx. MW: 68kDa/110kDa
Protein Family
Similar Products
Product Notes
The CDK9 (Catalog #AAA516729) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CDK9/CyclinT1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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