Mouse acrolein (ACR) ELISA Kit | ACR elisa kit
Mouse acrolein (ACR) ELISA Kit
Reactivity
Mouse
Synonyms
acrolein (ACR); Mouse acrolein (ACR) ELISA Kit; ACR elisa kit
Reactivity
Mouse
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive (Quantitative)
Sensitivity
0.1 ng/ml.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ACR elisa kit
Intended Uses: This ACR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACR. This ELISA kit for research use only!
Principle of the Assay: ACR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACR antibody and an ACR-HRP conjugate. The assay sample and buffer are incubated together with ACR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACR concentration since ACR from samples and ACR-HRP conjugate compete for the anti-ACR antibody binding site. Since the number of sites is limited, as more sites are occupied by ACR from the sample, fewer sites are left to bind ACR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACR concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ACR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACR antibody and an ACR-HRP conjugate. The assay sample and buffer are incubated together with ACR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACR concentration since ACR from samples and ACR-HRP conjugate compete for the anti-ACR antibody binding site. Since the number of sites is limited, as more sites are occupied by ACR from the sample, fewer sites are left to bind ACR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACR concentration in each sample is interpolated from this standard curve.
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