Activity
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USP12 recombinant protein
USP12, Active
Synonyms
USP12; Active; Ubiquitin specific peptidase 12; USP12 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.25mM DTT, 0.1mM EDTA, 25% glycerol.
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
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Activity
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SDS-Page
(Purity: Sample purity data. For lot-specific data, please refer to the specific datasheet.)
SDS-Page
(Purity: Sample purity data. For lot-specific data, please refer to the specific datasheet.)
Related Product Information for USP12 recombinant protein
Recombinant human full-length USP12 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_182488.
Usp12 belongs to the ubiquitin-specific protease family of deubiquitinases that contains the conserved catalytic cysteine/histidine domain present in other USPs. USP12 has been identified as a deubiquitinase for histones H2A/H2B, Notch, pHdomain leucine-rich repeat protein phosphatase 1, and androgen receptor (1). A complex of USP12 with WDR48 negatively regulates Akt activation, thus promoting cellular apoptosis. This complex has also been shown to suppress proliferation of tumor cell (2).
Usp12 belongs to the ubiquitin-specific protease family of deubiquitinases that contains the conserved catalytic cysteine/histidine domain present in other USPs. USP12 has been identified as a deubiquitinase for histones H2A/H2B, Notch, pHdomain leucine-rich repeat protein phosphatase 1, and androgen receptor (1). A complex of USP12 with WDR48 negatively regulates Akt activation, thus promoting cellular apoptosis. This complex has also been shown to suppress proliferation of tumor cell (2).
Product Categories/Family for USP12 recombinant protein
References
1. Joo, H. Y. et al. Regulation of histone H2A and H2B deubiquitination and Xenopus development by USP12 and USP46. J. Biol. Chem. 286:7190-7201, 2011.
2. Gangula, N.R. WD repeat protein WDR48 in complex with deubiquitinase USP12 suppresses Akt-dependent cell survival signaling by stabilizing pHdomain leucine-rich repeat protein phosphatase 1 (PHLPP1). J Biol Chem. 288(48):34545-34554, 2013.
2. Gangula, N.R. WD repeat protein WDR48 in complex with deubiquitinase USP12 suppresses Akt-dependent cell survival signaling by stabilizing pHdomain leucine-rich repeat protein phosphatase 1 (PHLPP1). J Biol Chem. 288(48):34545-34554, 2013.
NCBI and Uniprot Product Information
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Product Notes
The USP12 (Catalog #AAA517764) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "USP12, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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