Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
OTUD7B recombinant protein
OTUD7B, Active
Applications
Western Blot
Synonyms
OTUD7B; Active; CEZANNE; ZA20D1; OTU deubiquitinase 7B; OTUD7B recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Applicable Applications for OTUD7B recombinant protein
Activity Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for OTUD7B recombinant protein
Recombinant human OTUD7B (1-482) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_020205.
OUTD7B is a deubiquitinylase that belongs A20 subgroup of ovarian tumor (OUT) protein superfamily. It plays an essential role in inflammation and proliferation pathways. (1) OTUD7B is involved in regulation of the non-canonical NF-NF-kappaB pathway. (2) Studies suggest role of OUTD7B in cancer and has been shown to promote lung cancer cell proliferation and migration via Akt/VEGF signal pathway. Therefore, strategies to silence OTUD7B in carcinoma tissues may provide insights into developing therapies against lung adenocarcinoma and squamous carcinoma. (3)
OUTD7B is a deubiquitinylase that belongs A20 subgroup of ovarian tumor (OUT) protein superfamily. It plays an essential role in inflammation and proliferation pathways. (1) OTUD7B is involved in regulation of the non-canonical NF-NF-kappaB pathway. (2) Studies suggest role of OUTD7B in cancer and has been shown to promote lung cancer cell proliferation and migration via Akt/VEGF signal pathway. Therefore, strategies to silence OTUD7B in carcinoma tissues may provide insights into developing therapies against lung adenocarcinoma and squamous carcinoma. (3)
Product Categories/Family for OTUD7B recombinant protein
References
1. Cao L. et al. The N-terminal ubiquitin-associated domain of Cezanne is crucial for its function to suppress NF-kappaB pathway. J Cell Biochem. 119:1979-91 2018.
2. Hongbo H. et al. OTUD7B controls non-canonical NF-kappaB activation through deubiquitination of TRAF3. Nature. 494:371-374, 2013.
3. Dan-dan L. Upregulation of OTUD7B (Cezanne) promotes tumor progression via AKT/VEGF pathway in lung squamous carcinoma and adenocarcinoma. Frontiers in Oncology. 9:862, 2019.
2. Hongbo H. et al. OTUD7B controls non-canonical NF-kappaB activation through deubiquitination of TRAF3. Nature. 494:371-374, 2013.
3. Dan-dan L. Upregulation of OTUD7B (Cezanne) promotes tumor progression via AKT/VEGF pathway in lung squamous carcinoma and adenocarcinoma. Frontiers in Oncology. 9:862, 2019.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
81kDa
Protein Family
Similar Products
Product Notes
The OTUD7B (Catalog #AAA517353) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's OTUD7B can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Western Blot (WB). Researchers should empirically determine the suitability of the OTUD7B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OTUD7B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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