ULBP-3 recombinant protein

Recombinant human ULBP-3 protein

Cat. Num. AAA201322

• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: ULBP-3; Recombinant human ULBP-3 protein; NKG2D ligand 3; UL16 binding protein 3; N2DL-3; NKG2DL3; RAET1N; ALCAN-gamma; Retinoic acid early transcript 1N; ULBP-3 recombinant protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 30-217aa
• Sequence: DAHSLWY NFTIIHLPRH GQQWCEVQSQ VDQKNFLSYD CGSDKVLSMG HLEEQLYATD AWGKQLEMLR EVGQRLRLEL ADTELEDFTP SGPLTLQVRM SCECEADGYI RGSWQFSFDG RKFLLFDSNN RKWTVVHAGA RRMKEKWEKD SGLTTFFKMV SMRDCKSWLR DFLMHRKKRL EPTAPPTMAP G

• Applicable Applications for ULBP-3 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: hIgG-His-Tag
• Predicted N-term: Ala
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for ULBP-3 recombinant protein

ULBP-3, also known as NKG2D ligand 3, is ligands for natural-killer group 2, member D (NKG2D) receptor. It is related to MHC class I molecules, but its gene maps outside the MHC locus. The domain structure of ULBP3 differs significantly from those of conventional MHC class I molecules. It does not contain the alpha3 domain and the transmembrane segment. ULBP3 is thus composed of only the alpha1alpha2 domain which is linked to the cell membrane by the GPI anchor. It functions as a stress-induced ligand for NKG2D receptor. In the normal hair follicle, ULBP3 is turned off. However, different studies reported its high level in alopecia areata (AA). It is also expressed on some tumor cells and have been implicated in tumor surveillance. Recombinant human ULBP-3, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for ULBP-3 recombinant protein

Immunology; Proteins

References

Moftah NH, et al, (2016) Arch Dermatol Res. 308:415-421. Wirsching HG, et al, (2019) JCI Insight. 4:e128217.

NCBI and Uniprot Product Information

• UniProt Accession #: Q9BZM4
• Molecular Weight: 49.3kDa (430aa)
• UniProt Protein Name: NKG2D ligand 3
• UniProt Gene Name: ULBP3
• UniProt Synonym Gene Names: N2DL3; RAET1N; N2DL-3; NKG2DL3
• UniProt Entry Name: N2DL3_HUMAN

Uniprot Description

ULBP3: Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP2. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. Has lower affinity for NKG2D compared to ULBP1 and ULBP2 and induces weaker signaling responses than does ULBP2 or ULBP1. Belongs to the MHC class I family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: anchored to plasma membrane; plasma membrane

Molecular Function: antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; natural killer cell activation; natural killer cell mediated cytotoxicity; regulation of immune response