Monkey UL16 Binding protein 3 ELISA Kit | ULBP3 elisa kit

Monkey UL16 Binding protein 3 ELISA Kit

Cat. Num. AAA754928

• Gene Names: ULBP3; RAET1N
• Reactivity: Monkey
• Synonyms: UL16 Binding protein 3; Monkey UL16 Binding protein 3 ELISA Kit; ULBP3 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ULBP3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ULBP3. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This ULBP3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ULBP3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ULBP3 elisa kit

Principle of the Assay: ULBP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ULBP3 antibody and an ULBP3-HRP conjugate. The assay sample and buffer are incubated together with ULBP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ULBP3 concentration since ULBP3 from samples and ULBP3-HRP conjugate compete for the anti-ULBP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ULBP3 from the sample, fewer sites are left to bind ULBP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ULBP3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ULBP3 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 119568157
• NCBI GeneID: 79465
• Molecular Weight: 27,949 Da
• NCBI Official Full Name: UL16 binding protein 3
• NCBI Official Synonym Full Names: UL16 binding protein 3
• NCBI Official Symbol: ULBP3
• NCBI Official Synonym Symbols: RAET1N
• NCBI Protein Information: NKG2D ligand 3; N2DL-3; NKG2DL3; ALCAN-gamma; UL16-binding protein 3; retinoic acid early transcript 1N
• UniProt Protein Name: NKG2D ligand 3
• Protein Family: NKG2D ligand
• UniProt Gene Name: ULBP3
• UniProt Synonym Gene Names: N2DL3; RAET1N; N2DL-3; NKG2DL3
• UniProt Entry Name: N2DL3_HUMAN

Uniprot Description

ULBP3: Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP2. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. Has lower affinity for NKG2D compared to ULBP1 and ULBP2 and induces weaker signaling responses than does ULBP2 or ULBP1. Belongs to the MHC class I family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: anchored to plasma membrane; plasma membrane

Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; regulation of immune response; viral reproduction; natural killer cell mediated cytotoxicity; natural killer cell activation