Pim-1 Oncogene (PIM1) Recombinant Protein | PIM1 recombinant protein

Recombinant Pim-1 Oncogene (PIM1)

Cat. Num. AAA2009402

• Gene Names: Pim1; Pim-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Pim-1 Oncogene (PIM1); Recombinant Pim-1 Oncogene (PIM1); PIM1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-YQVGPLLGS GGFGSVYSGI RVADNLPVAI KHVEKDRISD WGELPNGTRV PMEVVLLKKV SSDFSGVIRL LDWFERPDSF VLILERPEPV QDLFDFITER GALQEDLARG FFWQVLEAVR HCHNCGVLHR DIKDENILID L
• Sequence Length: 397

• Applicable Applications for PIM1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Tyr122~Leu261 (Accession # P06803) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 83305340
• NCBI GeneID: 18712
• UniProt Accession #: P06803
• Molecular Weight: 17.4kDa
• NCBI Official Full Name: Serine/threonine-protein kinase pim-1
• NCBI Official Synonym Full Names: proviral integration site 1
• NCBI Official Symbol: Pim1
• NCBI Official Synonym Symbols: Pim-1
• NCBI Protein Information: serine/threonine-protein kinase pim-1; pim-1 oncogene; proto-oncogene serine/threonine-protein kinase pim-1
• UniProt Protein Name: Serine/threonine-protein kinase pim-1
• Protein Family: Protein
• UniProt Gene Name: Pim1
• UniProt Synonym Gene Names: Pim-1
• UniProt Entry Name: PIM1_MOUSE

Uniprot Description

Pim1: a proto-oncogene serine/threonine kinase involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerced by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl- X(L)/BCL2L1. Phosphorylation of ASK1 an other proapoptotic protein, by PIM1, significantly decreases ASK1 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of p21Cip1, a regulator of cell cycle progression at G1, results in the relocation of p21Cip1 to the cytoplasm and enhanced p21Cip1 protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, p27Kip1, at both transcriptional and post- translational levels. Phosphorylation of p27Kip1,induces 14-3-3- proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Binds to RP9. Isoform 1, but not isoform 2, binds BMX. Isoform 2 interacts with p27Kip1 and FOXO3. Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM1, ubiquitination and proteasomal degradation. Interacts with HSP90, this interaction stabilizes PIM1 protein levels. Ubiquitinated form interacts with HSP70 and promotes its proteosomal degradation. Strongly induced in leukocytes by the JAK/STAT pathway in response to cytokines. Induced by different cellular stresses, heat shock and cytotoxic agents. Expressed primarily in cells of the hematopoietic and germline lineages. 2 isoforms of the human protein are produced by alternative initiation. Both isoforms are expressed in prostate cancer cell lines.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Oncoprotein; CAMK group; PIM family

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; manganese ion binding; transcription factor binding; ribosomal small subunit binding; ATP binding

Biological Process: cell proliferation; negative regulation of transcription factor activity; protein amino acid autophosphorylation; regulation of mitotic cell cycle; protein amino acid phosphorylation; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; negative regulation of apoptosis