Human ULBP3 ELISA Kit | ULBP3 elisa kit

Human ULBP3 PicoKine ELISA Kit

Cat. Num. AAA1752560

• Gene Names: ULBP3; N2DL-3; RAET1N; NKG2DL3
• Reactivity: Human
• Synonyms: ULBP3; Human ULBP3 PicoKine ELISA Kit; Human ULBP3; UL16 binding protein 3; UL16-binding protein 3; ALCAN-gamma; NKG2D ligand 3; N2DL-3; NKG2DL3; Retinoic acid early transcript 1N; N2DL3; RAET1N; ULBP3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human ULBP3
• Sequence Length: 244

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ULBP3 elisa kit

Principle of the Assay: The Picokine™ Human ULBP3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human ULBP3 with a 96-well strip plate that is pre-coated with antibody specific for ULBP3. The detection antibody is a biotinylated antibody specific for ULBP3. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human ULBP3 with immunogen: Expression system for standard: NSO, Immunogen sequence: D30-G217. The kit is analytically validated with ready to use reagents. To measure Human ULBP3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human ULBP3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ULBP3 in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 13375656
• NCBI GeneID: 79465
• NCBI Accession #: NP_078794.1
• NCBI GenBank Nucleotide #: NP_078794.1
• UniProt Accession #: Q9BZM4
• NCBI Official Full Name: UL16-binding protein 3
• NCBI Official Synonym Full Names: UL16 binding protein 3
• NCBI Official Symbol: ULBP3
• NCBI Official Synonym Symbols: N2DL-3; RAET1N; NKG2DL3
• NCBI Protein Information: UL16-binding protein 3
• UniProt Protein Name: NKG2D ligand 3
• Protein Family: NKG2D ligand
• UniProt Gene Name: ULBP3
• UniProt Synonym Gene Names: N2DL3; RAET1N; N2DL-3; NKG2DL3
• UniProt Entry Name: N2DL3_HUMAN

NCBI Description

The protein encoded by this gene is one of several related ligands of the KLRK1/NKG2D receptor, which is found in primary NK cells. Binding of these ligands to the receptor activates several signal transduction pathways, including the JAK2, STAT5, and ERK pathways. The encoded protein is expressed solubly and on the surface of many tumor cells, making it potentially an important target for therapeutics. [provided by RefSeq, Nov 2015]

Uniprot Description

ULBP3: Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP2. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. Has lower affinity for NKG2D compared to ULBP1 and ULBP2 and induces weaker signaling responses than does ULBP2 or ULBP1. Belongs to the MHC class I family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: anchored to plasma membrane; plasma membrane

Molecular Function: antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; natural killer cell activation; natural killer cell mediated cytotoxicity; regulation of immune response