Typical Testing Data/Standard Curve (for reference only)
Mouse thyroid peroxidase ELISA Kit | TPO elisa kit
Mouse Thyroid-Peroxidase, TPO ELISA Kit
Reactivity
Mouse
Synonyms
thyroid peroxidase; Mouse Thyroid-Peroxidase; TPO ELISA Kit; MSA; TDH2A; TPX; OTTHUMP00000115536; thyroid microsomal antigen; thyroperoxidase; TPO elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse TPO. No significant cross-reactivity or interference between Mouse TPO and analogues was observed.
Sequence Length
914
Assay Type
Competitive
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Oxidative Stress
Protein Biological Process 3
Hydrogen peroxide
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TPO elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to TPO has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated TPO and incubated. A competitive inhibition reaction is launched between TPO (Standards or samples) and Biotin-conjugated TPO with the pre-coated antibody specific for TPO. The more amount of TPO in samples, the less antibody bound by Biotin-conjugated TPO. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of TPO in the sample. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101,342 Da
NCBI Official Full Name
thyroid peroxidase
NCBI Official Synonym Full Names
thyroid peroxidase
NCBI Official Symbol
Tpo
NCBI Protein Information
thyroid peroxidase
UniProt Protein Name
Thyroid peroxidase
Protein Family
UniProt Gene Name
Tpo
UniProt Synonym Gene Names
TPO
UniProt Entry Name
PERT_MOUSE
Uniprot Description
Function: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.
Catalytic activity: 2 iodide + H2O2 + 2 H+ = 2 iodine + 2 H2O.
Cofactor: Binds 1 calcium ion per heterodimer
Research Articles on TPO
Similar Products
Product Notes
The Mouse TPO tpo (Catalog #AAA702966) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA702966 ELISA Kit recognizes Mouse TPO. It is sometimes possible for the material contained within the vial of "thyroid peroxidase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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