Human myeloperoxidase ELISA Kit | MPO elisa kit

Human myeloperoxidase, MPO ELISA Kit

Cat. Num. AAA702138

• Reactivity: Human
• Synonyms: myeloperoxidase; Human myeloperoxidase; MPO ELISA Kit; MPO elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MPO. No significant cross-reactivity or interference between Human MPO and analogues was observed.
• Sequence Length: 745

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Detection Range: 6.25 ng/ml-400 ng/ml
• Sensitivity: 1.56 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Oxidative Stress
• Protein Biological Process 3: Hydrogen peroxide
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MPO elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MPO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MPO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557759
• NCBI GeneID: 4353
• NCBI Accession #: NP_000241.1
• NCBI GenBank Nucleotide #: NM_000250.1
• UniProt Accession #: P05164; Q14862; Q4PJH5; Q9UCL7; A1L4B8
• Molecular Weight: 83,869 Da
• NCBI Official Full Name: myeloperoxidase
• NCBI Official Synonym Full Names: myeloperoxidase
• NCBI Official Symbol: MPO
• NCBI Protein Information: myeloperoxidase
• UniProt Protein Name: Myeloperoxidase
• Protein Family: Myeloperoxidase
• UniProt Gene Name: MPO
• UniProt Synonym Gene Names: MPO
• UniProt Entry Name: PERM_HUMAN

NCBI Description

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Catalytic activity: Cl- + H2O2 + H+ = HClO + H2O.

Cofactor: Binds 1 calcium ion per monomer.