Testing Data
N6AMT2 recombinant protein
N6AMT2, Active
Gene Names
EEF1AKMT1; ESP13; N6AMT2
Applications
Methyltransferase Assay
Synonyms
N6AMT2; Active; N6AMT2 recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Sequence Positions
Full length
Sequence Length
932
Applicable Applications for N6AMT2 recombinant protein
Methyltransferase Assay
Species
Human
Tag
HIS tag
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for N6AMT2 recombinant protein
Description: Full-length recombinant human N6AMT2 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Scientific Background: N6AMT2 is a putative DNA methyltransferase, belongs to the methyltransferase superfamily, AML1 family. The N6AMT2 gene is conserved in human, mouse, rat, chimpanzee, dog, cow, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, N.crassa, A.thaliana, and rice (1). The N6AMT2 phosphorylation at Ser-2 has been reported (2). The function of this protein has not been widely studied yet.
Scientific Background: N6AMT2 is a putative DNA methyltransferase, belongs to the methyltransferase superfamily, AML1 family. The N6AMT2 gene is conserved in human, mouse, rat, chimpanzee, dog, cow, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, N.crassa, A.thaliana, and rice (1). The N6AMT2 phosphorylation at Ser-2 has been reported (2). The function of this protein has not been widely studied yet.
Product Categories/Family for N6AMT2 recombinant protein
References
1. Albrecht M, et.al: Novel Sm-like proteins with long C-terminal tails and associated methyltransferases. FEBS Lett. 2004 Jul 2;569 (1-3):18-26. 2. Gauci,S. et.al: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal. Chem. 81 (11), 4493-4501 (2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28 kDa
NCBI Official Full Name
Homo sapiens EEF1A lysine methyltransferase 1 (EEF1AKMT1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
EEF1A lysine methyltransferase 1
NCBI Official Symbol
EEF1AKMT1
NCBI Official Synonym Symbols
ESP13; N6AMT2
NCBI Protein Information
EEF1A lysine methyltransferase 1; protein-lysine N-methyltransferase N6AMT2
UniProt Protein Name
EEF1A lysine methyltransferase 1
Protein Family
UniProt Gene Name
EEF1AKMT1
Uniprot Description
EEF1AKMT1: a protein-lysine N-methyltransferase of the seven beta-strand class and AML1 family.
Protein type: EC 2.1.1.-; Methyltransferase
Chromosomal Location of Human Ortholog: 13q12.11
Cellular Component: cytosol
Molecular Function: methyltransferase activity; protein-lysine N-methyltransferase activity
Biological Process: peptidyl-lysine methylation; protein amino acid methylation
Research Articles on N6AMT2
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Product Notes
The N6AMT2 eef1akmt1 (Catalog #AAA515562) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full length. AAA Biotech's N6AMT2 can be used in a range of immunoassay formats including, but not limited to, Methyltransferase Assay. Researchers should empirically determine the suitability of the N6AMT2 eef1akmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N6AMT2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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