Bovine plasminogen ELISA Kit | PLG elisa kit

Bovine Plasminogen, PLG ELISA Kit

Cat. Num. AAA940842

• Gene Names: PLG; LPA
• Reactivity: Bovine
• Synonyms: plasminogen; Bovine Plasminogen; PLG ELISA Kit; Bovine Plasminogen (PLG) ELISA kit; RP1-81D8.1; DKFZp779M0222; plasmin; PLG elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bovine PLG. No significant cross-reactivity or interference between Bovine PLG and analogues was observed.
• Sequence Length: 812

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 78 ng/ml-5000 ng/ml
• Sensitivity: 19.5 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Blood Coagulation
• Protein Biological Process 3: Blood coagulation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLG elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with plasminogen. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for plasminogen. The competitive inhibition reaction is launched between with pre-coated plasminogen and plasminogen in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of plasminogen in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 27806815
• NCBI GeneID: 280897
• NCBI Accession #: NP_776376.1
• NCBI GenBank Nucleotide #: NM_173951.2
• UniProt Accession #: P06868; Q28162
• Molecular Weight: 91,216 Da
• NCBI Official Full Name: plasminogen
• NCBI Official Symbol: PLG
• NCBI Official Synonym Symbols: LPA
• NCBI Protein Information: plasminogen; lipoprotein, Lp(a)
• UniProt Protein Name: Plasminogen
• Protein Family: Plasminogen
• UniProt Gene Name: PLG
• UniProt Entry Name: PLMN_BOVIN

Uniprot Description

Function: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells

Catalytic activity: Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa; higher selectivity than trypsin. Converts fibrin into soluble products.

Enzyme regulation: Converted into plasmin by plasminogen activators, both plasminogen and its activator being bound to fibrin. Cannot be activated with streptokinase.