Rat thyroglobulin ELISA Kit | TG elisa kit

Rat Thyroglobulin, TG ELISA Kit

Cat. Num. AAA704121

• Gene Names: TG; TGN; AITD3
• Reactivity: Rat
• Synonyms: thyroglobulin; Rat Thyroglobulin; TG ELISA Kit; AITD3; TGN; ; TG elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat TG. No significant cross-reactivity or interference between Rat TG and analogues was observed.
• Sequence Length: 2,768

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 10 ng/ml-160 ng/ml
• Sensitivity: 10 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Biosynthesis/Metabolism
• Protein Biological Process 3: Thyroid hormones biosynthesis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TG elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TG. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated TG. A competitive inhibition reaction is launched between TG (Standards or samples) and Biotin-conjugated TG with the pre-coated antibody specific for TG. The more amount of TG in samples, the less antibody bound by Biotin-conjugated TG. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of TG in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 37174
• NCBI GeneID: 7038
• UniProt Accession #: P01266; Q9JKY6; Q9JM94
• Molecular Weight: 304,645 Da
• NCBI Official Full Name: thyroglobulin
• NCBI Official Synonym Full Names: thyroglobulin
• NCBI Official Symbol: TG
• NCBI Official Synonym Symbols: TGN; AITD3
• NCBI Protein Information: thyroglobulin
• UniProt Protein Name: Thyroglobulin
• Protein Family: Thyroglobulin
• UniProt Gene Name: Tg
• UniProt Synonym Gene Names: Tg
• UniProt Entry Name: THYG_RAT

NCBI Description

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]

Uniprot Description

Function: Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).