DSN1 sirna

DSN1 siRNA (Human)

Cat. Num. AAA8228168

• Gene Names: DSN1; KNL3; MIS13; hKNL-3; C20orf172; dJ469A13.2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DSN1; DSN1 siRNA (Human); C20orf172; MIS13; Kinetochore-associated protein DSN1 homolog; DSN1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DSN1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 356

• Applicable Applications for DSN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DSN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DSN1 sirna

siRNA to inhibit DSN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 223890147
• NCBI GeneID: 79980
• NCBI Accession #: NP_001138787.1
• NCBI GenBank Nucleotide #: NM_001145315.1
• UniProt Accession #: Q9H410; Q5JW55; Q5JW56; Q9H8P4; B4DWT2; E1P5U9
• Molecular Weight: 27,998 Da
• NCBI Official Full Name: kinetochore-associated protein DSN1 homolog isoform 1
• NCBI Official Synonym Full Names: DSN1 homolog, MIS12 kinetochore complex component
• NCBI Official Symbol: DSN1
• NCBI Official Synonym Symbols: KNL3; MIS13; hKNL-3; C20orf172; dJ469A13.2
• NCBI Protein Information: kinetochore-associated protein DSN1 homolog
• UniProt Protein Name: Kinetochore-associated protein DSN1 homolog
• Protein Family: Kinetochore-associated protein
• UniProt Gene Name: DSN1
• UniProt Synonym Gene Names: C20orf172; MIS13
• UniProt Entry Name: DSN1_HUMAN

NCBI Description

This gene encodes a kinetochore protein that functions as part of the minichromosome instability-12 centromere complex. The encoded protein is required for proper kinetochore assembly and progression through the cell cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]

Uniprot Description

DSN1: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: cytoplasm; nucleus; cytosol; MIS12/MIND type complex

Molecular Function: protein binding

Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation