Rabbit anti-Mouse, Rat GRM1 Polyclonal Antibody | anti-GRM1 antibody

Anti-GRM1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103440

• Gene Names: Grm1; rcw; wobl; Gprc1a; mGluR1; nmf373; Gm10828; 4930455H15Rik; ENSMUSG00000075319
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: GRM1; Polyclonal Antibody; Anti-GRM1 Antibody; Rabbit Polyclonal; GRM1 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-Gprc1a Antibody; Anti-Mglur1 Antibody; anti-GRM1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse GRM1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GRM1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse GRM1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse GRM1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining GRM1 in mouse stomach with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining GRM1 in rat stomach with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-GRM1 antibody

GRM1 gene, which is located on 6q24.3, encodes the metabotropic glutamate receptor type 1 (mGluR1), a transmembrane protein highly expressed in cerebellar Purkinje cells. Pathogenic variants in GRM1 have been reported only three times in humans, causing autosomal-recessive cerebellar ataxia with an early-onset and intellectual disability or dominant forms of cerebellar ataxia with a less severe phenotype in adults. GRM1 cause a recessive form of cerebellar ataxia, SCAR13. A neuronal receptor, GRM1, when ectopically expressed in melanocytes, induces in vitro melanocytic transformation and spontaneous malignant melanoma development in vivo in a transgenic mouse model. GRM1 signaling has been implicated in benign and malignant disorders including prostate cancer (PCa). GRM1 encodes the metabotropic glutamate receptor 1 (mGluR1), whose documented role as a modulator of neuronal signaling and synaptic plasticity makes it a plausible schizophrenia candidate.

References

Wangari-Talbot J, et al. (2012) Functional effects of grm1 suppression in human melanoma cells. Mol Cancer Res 10 (11): 1440-1450.Wall BA, et al. (2014) Disruption of grm1-mediated signalling using riluzole results in DNA damage in melanoma cells. Pigment Cell Melanoma Res 27 (2): 263-274.Isola AL, et al. (2018) Exosomes released by metabotropic glutamate receptor 1 (grm1) expressing melanoma cells increase cell migration and invasiveness. Oncotarget 9 (1): 1187-1199.Cabet S, et al. (2019) A novel truncating variant p.(arg297*) in the grm1 gene causing autosomal-recessive cerebellar ataxia with juvenile-onset. Eur J Med Genet 62 (10): 103726.

NCBI and Uniprot Product Information

• NCBI GI #: 50927519
• NCBI GeneID: 14816
• UniProt Accession #: P97772; Q6AXG4; Q9EPV6
• Molecular Weight: 133,212 Da
• NCBI Official Full Name: Grm1 protein
• NCBI Official Synonym Full Names: glutamate receptor, metabotropic 1
• NCBI Official Symbol: Grm1
• NCBI Official Synonym Symbols: rcw; wobl; Gprc1a; mGluR1; nmf373; Gm10828; 4930455H15Rik; ENSMUSG00000075319
• NCBI Protein Information: metabotropic glutamate receptor 1; G protein coupled receptor, family C, group 1, member A; G protein-coupled receptor, family C, group 1, member A
• UniProt Protein Name: Metabotropic glutamate receptor 1
• Protein Family: Metabotropic glutamate receptor
• UniProt Gene Name: Grm1
• UniProt Synonym Gene Names: Gprc1a; Mglur1; mGluR1
• UniProt Entry Name: GRM1_MOUSE

Uniprot Description

mGluR1: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol- calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP. Belongs to the G-protein coupled receptor 3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 3; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: neuron projection; integral to plasma membrane; dendrite; postsynaptic density; dendritic spine; integral to membrane; cytosol; presynaptic membrane; postsynaptic membrane; cell soma; membrane; axon; plasma membrane; intracellular; nucleus

Molecular Function: G-protein coupled receptor activity; identical protein binding; signal transducer activity; protein binding; PLC activating metabotropic glutamate receptor activity; estrogen receptor binding; glutamate receptor activity

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of synaptic transmission, glutamatergic; activation of MAPKK activity; activation of MAPK activity; calcium-mediated signaling; locomotory behavior; signal transduction; sensory perception of pain; G-protein coupled receptor protein signaling pathway; regulation of MAPKKK cascade; metabotropic glutamate receptor, phospholipase C activating pathway; protein kinase C activation; metabotropic glutamate receptor signaling pathway; regulation of sensory perception of pain