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Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.)

Rabbit ADD1 Polyclonal Antibody | anti-ADD1 antibody

ADD1 (Phospho-Ser726) Antibody

Gene Names
ADD1; ADDA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
ADD1; Polyclonal Antibody; ADD1 (Phospho-Ser726) Antibody; Alpha-adducin; Erythrocyte adducin subunit alpha; ADDA; anti-ADD1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
ADD1 (Phospho-Ser726) Antibody detects endogenous levels of ADD1 only when phosphorylated at Ser726.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
662
Applicable Applications for anti-ADD1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:40000
Target Modification
Phospho
Modification Sites
H:S726/S713; M:S724/S712; R:S724/S712
Immunogen
The antiserum was produced against synthesized peptide derived from human ADD1 around the phosphorylation site of Ser726.
Immunogen Range
688-737
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from HeLa cells treated with Forskolin 40nM 30', using ADD1 (Phospho-Ser726) Antibody. The lane on the right is blocked with the phospho peptide.)

Western Blot (WB) (Western blot analysis of lysates from HeLa cells treated with Forskolin 40nM 30', using ADD1 (Phospho-Ser726) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
118
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80 kDa
NCBI Official Full Name
alpha-adducin isoform a
NCBI Official Synonym Full Names
adducin 1
NCBI Official Symbol
ADD1
NCBI Official Synonym Symbols
ADDA
NCBI Protein Information
alpha-adducin
UniProt Protein Name
Alpha-adducin
Protein Family
UniProt Gene Name
ADD1
UniProt Synonym Gene Names
ADDA

NCBI Description

Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017]

Uniprot Description

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Research Articles on ADD1

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Product Notes

The ADD1 add1 (Catalog #AAA9502320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADD1 (Phospho-Ser726) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000. Researchers should empirically determine the suitability of the ADD1 add1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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