Rabbit anti-Human MKS1 Polyclonal Antibody | anti-MKS1 antibody

MKS1 Antibody (N-Term)

Cat. Num. AAA9231277

• Gene Names: MKS1; MES; MKS; BBS13; POC12; JBTS28
• Reactivity: Human
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot, ELISA
• Purity: Purified through a protein A column, followed by peptide affinity purification.
• Synonyms: MKS1; Polyclonal Antibody; MKS1 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Meckel syndrome type 1 protein; anti-MKS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This MKS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 90-124 amino acids from the human region of human MKS1.
• Purity/Purification: Purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 549

• Applicable Applications for anti-MKS1 antibody: Immunofluorescence (IF), Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA)
• Application Notes: IF: 1:25; FC: 1:25; WB: 1:2000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Cellular Location: Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localizes at the transition zone, a region between the basal body and the ciliary axoneme.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks.; For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with AP22330a at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).)

Flow Cytometry (FC/FACS)

(Overlay histogram showing HepG2 cells stained with AP22330a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22330a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

Western Blot (WB)

(All lanes : Anti-MKS1 Antibody (N-Term) at 1:2000 dilutionLane 1: Human kidney lysateLane 2: Human lung lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MKS1 antibody

Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in centrosome migration to the apical cell surface during early ciliogenesis. Required for ciliary structure and function, including a role in regulating length and appropriate number through modulating centrosome duplication. Required for cell branching morphology.

Product Categories/Family for anti-MKS1 antibody

Primary Antibodies

References

Kyttaelae M.,et al.Nat. Genet. 38:155-157(2006). Ota T.,et al.Nat. Genet. 36:40-45(2004). Zody M.C.,et al.Nature 440:1045-1049(2006). Ebert L.,et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Dawe H.R.,et al.Hum. Mol. Genet. 16:173-186(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 260064077
• NCBI GeneID: 54903
• NCBI Accession #: NP_001159399.1
• NCBI GenBank Nucleotide #: NM_001165927.1
• UniProt Accession #: Q9NXB0; Q284T0; Q96G13; B7WNX4; F5H885
• Molecular Weight: 64528
• NCBI Official Full Name: Meckel syndrome type 1 protein isoform 2
• NCBI Official Synonym Full Names: Meckel syndrome, type 1
• NCBI Official Symbol: MKS1
• NCBI Official Synonym Symbols: MES; MKS; BBS13; POC12; JBTS28
• NCBI Protein Information: Meckel syndrome type 1 protein
• UniProt Protein Name: Meckel syndrome type 1 protein
• Protein Family: Meckel syndrome type 1 protein
• UniProt Gene Name: MKS1

NCBI Description

The protein encoded by this gene localizes to the basal body and is required for formation of the primary cilium in ciliated epithelial cells. Mutations in this gene result in Meckel syndrome type 1 and in Bardet-Biedl syndrome type 13. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in centrosome migration to the apical cell surface during early ciliogenesis. Required for ciliary structure and function, including a role in regulating length and appropriate number through modulating centrosome duplication. Required for cell branching morphology.