Signal Transducer And Activator Of Transcription 6 (STAT6) Recombinant Protein | STAT6 recombinant protein

Recombinant Signal Transducer And Activator Of Transcription 6 (STAT6)

Cat. Num. AAA2011871

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Signal Transducer And Activator Of Transcription 6 (STAT6); Recombinant Signal Transducer And Activator Of Transcription 6 (STAT6); STAT6 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-L VVIVHGNQDN NAKATILWDN AFSEMDRVPF VVAERVPWEK MCETLNLKFM AEVGTSRGLL PEHFLFLAQK IFNDNSLSVE AFQHRCVSWS QFNKEILLGR GFTFWQWFDG VLDLTKRCLR SYWSDRLIIG FISKQYVTSL LLNEPDGTFL LRFSDSEIGG ITIAHVIRGQ DGSSQIENIQ PFSAKDLSIR SLGDRIRDLA QLKNLYPKKP KDE
• Sequence Length: 837

• Applicable Applications for STAT6 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu410~Glu623 (Accession # P52633) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 128485774
• NCBI GeneID: 20852
• NCBI Accession #: NP_033310.2
• NCBI GenBank Nucleotide #: NM_009284.2
• UniProt Accession #: P52633; Q8R2F1
• Molecular Weight: 28.4kDa
• NCBI Official Full Name: signal transducer and transcription activator 6
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 6
• NCBI Official Symbol: Stat6
• NCBI Protein Information: signal transducer and transcription activator 6
• UniProt Protein Name: Signal transducer and transcription activator 6
• Protein Family: Signal transducer and transcription activator
• UniProt Gene Name: Stat6
• UniProt Entry Name: STAT6_MOUSE

Uniprot Description

STAT6: transcription factor of the STAT family. Plays a central role in IL4-mediated biological responses. Induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. May function in the differentiation of T helper 2 cells and class switch of immunoglobulins. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; nucleus; lipid raft

Molecular Function: identical protein binding; signal transducer activity; DNA binding; sequence-specific DNA binding; protein phosphatase binding; transcription factor activity

Biological Process: T-helper 1 cell lineage commitment; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of T-helper 2 type immune response; response to cytokine stimulus; cytokine and chemokine mediated signaling pathway; mammary gland epithelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of isotype switching to IgE isotypes; regulation of cell proliferation