Fas Associating Death Domain Containing Protein (FADD) Recombinant Protein | FADD recombinant protein

Recombinant Fas Associating Death Domain Containing Protein (FADD)

Cat. Num. AAA2009820

• Gene Names: Fadd; Mort1/FADD
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fas Associating Death Domain Containing Protein (FADD); Recombinant Fas Associating Death Domain Containing Protein (FADD); FADD recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LLHS LSGSLSGNDL MELKFLCRER VSKRKLERVQ SGLDLFTVLL EQNDLERGHT GLLRELLASL RRHDLLQRLD DFEAGTATAA PPGEADLQVA FDIVCDNVGR DWKRLARELK VSEAKMDGIE EKYPRSLSER VRESLKVWKN AEKKNASVAG LVKALRTCRL NLVADLVE
• Sequence Length: 205

• Applicable Applications for FADD recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu7~Glu178 (Accession # Q61160) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6753812
• NCBI GeneID: 14082
• NCBI Accession #: NP_034305.1
• NCBI GenBank Nucleotide #: NM_010175.5
• UniProt Accession #: Q61160; Q3TC37; Q61082
• Molecular Weight: 23.2kDa
• NCBI Official Full Name: protein FADD
• NCBI Official Synonym Full Names: Fas (TNFRSF6)-associated via death domain
• NCBI Official Symbol: Fadd
• NCBI Official Synonym Symbols: Mort1/FADD
• NCBI Protein Information: protein FADD; FAS-associated death domain protein; mediator of receptor induced toxicity; Fas-associating protein with death domain; FAS-associating death domain-containing protein
• UniProt Protein Name: Protein FADD
• Protein Family: Fas-associated death domain protein
• UniProt Gene Name: Fadd
• UniProt Synonym Gene Names: Mort1
• UniProt Entry Name: FADD_MOUSE

Uniprot Description

FADD: an adaptor molecule that mediates apoptosis. Recruited through its C-terminal death domain by Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TRAIL-receptor, participating in the death signaling initiated by these receptors. Interaction with the receptors unmasks the N-terminal effector domain of this protein, which recruits caspase-8, and thereby activates the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Protein type: Adaptor/scaffold; Apoptosis

Cellular Component: neuron projection; protein complex; cytoplasm; CD95 death-inducing signaling complex; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; protease binding; death receptor binding; protein complex binding; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: cardiac muscle development; spleen development; thymus development; protein heterooligomerization; positive regulation of proteolysis; apoptosis; positive regulation of T cell mediated cytotoxicity; positive regulation of apoptosis; immune system process; T cell differentiation in the thymus; signal transduction; positive regulation of tumor necrosis factor production; positive regulation of activated T cell proliferation; lymph node development; positive regulation of interleukin-8 production; regulation of apoptosis; positive regulation of interferon-gamma production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; T cell homeostasis; positive regulation of macrophage differentiation; defense response to virus; positive regulation of adaptive immune response; T cell differentiation