Rabbit anti-Human, Mouse XDH Polyclonal Antibody | anti-XDH antibody

XDH, NT (XDH, XDHA, Xanthine dehydrogenase/oxidase, Xanthine dehydrogenase, Xanthine oxidase, Xanthine oxidoreductase) (AP)

Cat. Num. AAA6363929

• Gene Names: XDH; XO; XOR
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: XDH; Polyclonal Antibody; NT (XDH; XDHA; Xanthine dehydrogenase/oxidase; Xanthine dehydrogenase; Xanthine oxidase; Xanthine oxidoreductase) (AP); Xanthine oxidoreductase; anti-XDH antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human XDH. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-XDH antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 206-234 in the N-terminal region of human XDH.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MBS6009226 in mouse lung tissue lysates (35ug/lane). XDH (arrow) was detected usingMBS6009226.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted withMBS6009226, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6009226 for immunohistochemistry; clinical relevance has not been evaluated.)

Testing Data

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Product Categories/Family for anti-XDH antibody

Antibodies; Enzymes; Oxidase

References

1. Ross, C.J., et.al., Nat. Genet. 41(12):1345-1349 (2009). 2. Taibi, G., et.al., J. Cell. Biochem. 108(3):688-692 (2009). 3. Spiekermann, S., et.al., Eur. Respir. J. 34(1):276 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 91823271
• NCBI GeneID: 7498
• NCBI Accession #: NP_000370.2
• NCBI GenBank Nucleotide #: NM_000379.3
• UniProt Accession #: P47989; Q16681; Q16712; Q4PJ16
• Molecular Weight: 146,424 Da
• NCBI Official Full Name: xanthine dehydrogenase/oxidase
• NCBI Official Synonym Full Names: xanthine dehydrogenase
• NCBI Official Symbol: XDH
• NCBI Official Synonym Symbols: XO; XOR
• NCBI Protein Information: xanthine dehydrogenase/oxidase; xanthine oxidoreductase
• UniProt Protein Name: Xanthine dehydrogenase/oxidase
• Protein Family: Xanthine dehydrogenase
• UniProt Gene Name: XDH
• UniProt Synonym Gene Names: XDHA; XD; XO; XOR
• UniProt Entry Name: XDH_HUMAN

NCBI Description

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]

Uniprot Description

XDH: Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). Defects in XDH are the cause of xanthinuria type 1 (XU1). Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. Belongs to the xanthine dehydrogenase family.

Protein type: Secondary Metabolites Metabolism - caffeine; EC 1.17.1.4; EC 1.17.3.2; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 2p23.1

Cellular Component: extracellular space; peroxisome; cytosol

Molecular Function: molybdopterin cofactor binding; 2 iron, 2 sulfur cluster binding; UDP-N-acetylmuramate dehydrogenase activity; protein homodimerization activity; oxidoreductase activity, acting on the aldehyde or oxo group of donors; electron carrier activity; FAD binding; iron ion binding; xanthine dehydrogenase activity; xanthine oxidase activity

Biological Process: caspase activation; lactation; xanthine catabolic process; negative regulation of protein kinase B signaling cascade; negative regulation of endothelial cell differentiation; negative regulation of protein amino acid phosphorylation; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of endothelial cell proliferation; purine nucleotide catabolic process; purine base metabolic process

Disease: Xanthinuria, Type I