Metallothionein 3 (MT3) Recombinant Protein | MT3 recombinant protein

Recombinant Metallothionein 3 (MT3)

Cat. Num. AAA2018780

• Gene Names: Mt3; Mt-3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Metallothionein 3 (MT3); Recombinant Metallothionein 3 (MT3); MT3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDPETCPCPT GGSCTCSDKCKCKGCKCTNC KKSCCSCCPA GCEKCAKDCV CKGEEGAKAE AEKCSCCQ
• Sequence Length: 68

• Applicable Applications for MT3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Gln68 (Accession # P28184) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 7305287
• NCBI GeneID: 17751
• NCBI Accession #: NP_038631.1
• NCBI GenBank Nucleotide #: NM_013603.2
• UniProt Accession #: P28184
• Molecular Weight: 10.8kDa
• NCBI Official Full Name: metallothionein-3
• NCBI Official Synonym Full Names: metallothionein 3
• NCBI Official Symbol: Mt3
• NCBI Official Synonym Symbols: Mt-3
• NCBI Protein Information: metallothionein-3; GIF; MT-III; metallothionein-III; growth inhibitory factor
• UniProt Protein Name: Metallothionein-3
• Protein Family: Metallothionein
• UniProt Gene Name: Mt3
• UniProt Synonym Gene Names: MT-3; GIF; MT-III
• UniProt Entry Name: MT3_MOUSE

Uniprot Description

MT3: Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro. Belongs to the metallothionein superfamily. Type 1 family.

Cellular Component: synaptic vesicle; cell

Molecular Function: zinc ion binding; metal ion binding

Biological Process: positive regulation of catalytic activity; negative regulation of autophagy; positive regulation of transcription, DNA-dependent; cellular zinc ion homeostasis; zinc ion transport; regulation of protein amino acid glycosylation; astrocyte development; leptin-mediated signaling pathway; negative regulation of neurogenesis; cadmium ion homeostasis; cellular metal ion homeostasis; cellular lipid catabolic process; energy reserve metabolic process; negative regulation of neuron apoptosis; response to oxidative stress; negative regulation of transcription, DNA-dependent; cholesterol catabolic process; regulation of response to food; negative regulation of apoptosis