High Mobility Group AT Hook Protein 2 (HMGA2) Recombinant Protein | HMGA2 recombinant protein

Recombinant High Mobility Group AT Hook Protein 2 (HMGA2)

Cat. Num. AAA2011078

• Gene Names: Hmga2; pg; Hmgic; pygmy; HMGI-C; 9430083A20Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: High Mobility Group AT Hook Protein 2 (HMGA2); Recombinant High Mobility Group AT Hook Protein 2 (HMGA2); HMGA2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MSARGEGAGQ PSTSAQGQPA APVPQKRGRG RPRKQQQEPT CEPSPKRPRG RPKGSKNKSP SKAAQKKAET IGEKRPRGRP RKWPQQVVQK KPAQETEETS SQESAEED
• Sequence Length: 108

• Applicable Applications for HMGA2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Asp108 (Accession # P52927) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6754210
• NCBI GeneID: 15364
• NCBI Accession #: NP_034571.1
• NCBI GenBank Nucleotide #: NM_010441.2
• UniProt Accession #: P52927; Q3UQW0
• Molecular Weight: 15.6kDa
• NCBI Official Full Name: high mobility group protein HMGI-C
• NCBI Official Synonym Full Names: high mobility group AT-hook 2
• NCBI Official Symbol: Hmga2
• NCBI Official Synonym Symbols: pg; Hmgic; pygmy; HMGI-C; 9430083A20Rik
• NCBI Protein Information: high mobility group protein HMGI-C; high mobility group AT-hook protein 2; high mobility group protein I, isoform C
• UniProt Protein Name: High mobility group protein HMGI-C
• Protein Family: High mobility group protein
• UniProt Gene Name: Hmga2
• UniProt Synonym Gene Names: Hmgic
• UniProt Entry Name: HMGA2_MOUSE

Uniprot Description

HMGA2: Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Interacts with E4F1. Interacts with NEK2. Belongs to the HMGA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Oncoprotein

Cellular Component: nuclear chromosome; chromatin; nucleus

Molecular Function: protein binding; enzyme binding; DNA-(apurinic or apyrimidinic site) lyase activity; DNA binding; AT DNA binding

Biological Process: transcription from RNA polymerase II promoter; somatic stem cell maintenance; cell proliferation in forebrain; negative regulation of transcription from RNA polymerase II promoter; positive regulation of multicellular organism growth; signal transduction; regulation of growth hormone secretion; meiotic cell cycle; regulation of transcription, DNA-dependent; base-excision repair; regulation of growth; positive regulation of cell proliferation; mitosis; negative regulation of steroid hormone receptor signaling pathway; transcription, DNA-dependent; adrenal gland development; male gonad development; cell cycle; DNA catabolic process, endonucleolytic; chromosome condensation; cell proliferation; cell division; pituitary gland development; negative regulation of astrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; spermatogenesis; negative regulation of JAK-STAT cascade