Sprouty Homolog 1 (SPRY1) Recombinant Protein | SPRY1 recombinant protein

Recombinant Sprouty Homolog 1 (SPRY1)

Cat. Num. AAA2010724

• Gene Names: Spry1; sprouty1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Sprouty Homolog 1 (SPRY1); Recombinant Sprouty Homolog 1 (SPRY1); SPRY1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GSAASSGSS SSVSSEQGLL GRSPPTRPIP GHRSDRVIRT QPKQLLVEDL KASLKEDPTQ HKFICEQCGK CKCGECTAPR ALPSCLACDR QCLCSAESMV EYGTCMCLVK GIFYHCSNDD DGGSYSDNPC SCSQSHCCSR YLCMGALSLC LPCLLCYPPA KGCLKLCRGC YDWTHRPGCR CRNSNTVYCK LES
• Sequence Length: 313

• Applicable Applications for SPRY1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gly112~Ser303 (Accession # Q9QXV9) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6755650
• NCBI GeneID: 24063
• NCBI Accession #: NP_036026.1
• NCBI GenBank Nucleotide #: NM_011896.2
• UniProt Accession #: Q9QXV9
• Molecular Weight: 24.6kDa
• NCBI Official Full Name: protein sprouty homolog 1
• NCBI Official Synonym Full Names: sprouty homolog 1 (Drosophila)
• NCBI Official Symbol: Spry1
• NCBI Official Synonym Symbols: sprouty1
• NCBI Protein Information: protein sprouty homolog 1; spry-1; sprouty 1; inhibitor of receptor tyrosine kinases
• UniProt Protein Name: Protein sprouty homolog 1
• Protein Family: Protein sprouty
• UniProt Gene Name: Spry1
• UniProt Synonym Gene Names: Spry-1
• UniProt Entry Name: SPY1_MOUSE

Uniprot Description

SPRY1: May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Belongs to the sprouty family. Induced by FGF signaling.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis; Inhibitor

Cellular Component: membrane; cell; cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of cell proliferation; negative regulation of MAP kinase activity; establishment of mitotic spindle orientation; negative regulation of nerve growth factor receptor signaling pathway; ureteric bud development; negative regulation of fibroblast growth factor receptor signaling pathway; multicellular organismal development; induction of an organ; regulation of signal transduction; negative regulation of Ras protein signal transduction; metanephros development