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792 results for " STAT3 Antibody" - showing 1-50

Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using Phospho-STAT3-Y705 antibody (MBS125978) at 1:500 dilution.HeLa cells were treated by IFN-α (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.)

Stat3-Y705, Antibody (Cat# AAA125978)

Full Name
Phospho-Stat3-Y705 Polyclonal Antibody
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from normal (control) and STAT3 knockout (KO) HeLa cells, using STAT3 antibody (A1192) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s)

STAT3, Polyclonal Antibody (Cat# AAA127578)

Full Name
STAT3 Polyclonal Antibody
Gene Names
STAT3; APRF; HIES
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
$210/0.02 mL | $265/0.05 mL | $380/0.1 mL | $540/0.2 mL | $2,285/5x0.2 mL
Quality Control (Western blot analysis of immunized recombinant protein, using anti-STAT3 monoclonal antibody. )

STAT3, Monoclonal Antibody (Cat# AAA120037)

Full Name
Mouse monoclonal antibody Anti-Human STAT3
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human
Applications
EIA, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB) (Western blot detection of EGFR in A549, MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.)

EGF Receptor, Monoclonal Antibody (Cat# AAA475155)

Full Name
Anti-EGF Receptor Mouse mAb
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Monkey
Applications
WB, ICC, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot detection of EGFR in A549 and COS7 cell lysates using EGFR mouse mAb (dilution 1:2000).Predicted band size:134 Kda.Observed band size:175KDa.)

EGF Receptor, Monoclonal Antibody (Cat# AAA475211)

Full Name
Anti-EGF Receptor Mouse mAb
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Monkey
Applications
WB, ICC, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL

STAT3, ELISA Kit (Cat# AAA9501569)

Full Name
Phospho-STAT3 (Tyr705) Colorimetric Cell-Based ELISA Kit
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Pricing
$575/2x96 Wells | $2,580/10x96 Wells | $5,065/20x96 Wells
Western Blot (WB) (All lanes : Anti-SOCS1 Antibody (N-term) at 1:2000 dilutionLane 1: mouse thymus lysatesLane 2: rat spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

SOCS1, Polyclonal Antibody (Cat# AAA9210758)

Full Name
SOCS1 Antibody (N-term)
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
Mouse, rat
Applications
WB, EIA, IHC, IF, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL

KIT, Monoclonal Antibody (Cat# AAA9200373)

Full Name
KIT Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human
Applications
WB, EIA, IF
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of STAT3 (pY705) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

STAT3, Polyclonal Antibody (Cat# AAA8203504)

Full Name
Anti-STAT3 (pY705) Phospho Antibody
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human, Mouse, Rat, Bovine, Dog, Porcine, Rabbit, Sheep
Applications
WB, IHC, IP
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$185/0.03 mL | $275/0.1 mL | $380/0.2 mL | $1,650/5x0.2 mL
Western Blot (WB) (Western blot detection of PKR in Jurkat, A549 and MCF7 cell lysates using PKR mouse mAb (1:1000 diluted).Predicted band size:62KDa.Observed band size:74KDa.)

PKR, Monoclonal Antibody (Cat# AAA476586)

Full Name
Anti-PKR Mouse mAb
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human
Applications
WB
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with STAT3 Mouse Monoclonal Antibody (STAT3/2409).)

STAT3/Signal Transducer and Activator of Transcription 3, Monoclonal Antibody (Cat# AAA4380497)

Full Name
STAT3/Signal Transducer and Activator of Transcription 3
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human. Others not tested.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver tissue using MBS7045062 at dilution 1:100)

STAT3, Polyclonal Antibody (Cat# AAA7045062)

Full Name
STAT3 Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human
Applications
EIA, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunohistochemistry (IHC) (MBS9200563 staining CSF1R in human skin sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as the secondary antibody.)

CSF1R, Monoclonal Antibody (Cat# AAA9200563)

Full Name
CSF1R Antibody
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
WB, EIA, FC, IHC-P
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysates from Hela,CEM,mouse NIH/3T3 cell line (from left to right), using Stat3 Antibody. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)

STAT3, Monoclonal Antibody (Cat# AAA9231648)

Full Name
STAT3 Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse
Predicted: Pig, Rat
Applications
WB, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT3 (Phospho-Tyr705) Antibody. The picture on the right is blocked with the phospho peptide.)

STAT3, Polyclonal Antibody (Cat# AAA9503167)

Full Name
STAT3 (Phospho-Tyr705) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IP, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from Hela cells untreated or treated with IFN a using STAT3(Phospho-Tyr705) Antibody.)

STAT3, Polyclonal Antibody (Cat# AAA9411885)

Full Name
STAT3 (Phospho-Tyr705) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB) (Western blot-Phospho-Stat3-S727 pAb)

Stat3-S727, Polyclonal Antibody Pair Kit (Cat# AAA9135709)

Full Name
Phospho-Stat3-S727 Antibody Kit
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB
Pricing
$275/0.02 mL + 0.02 mL | $360/0.05 mL + 0.05 mL | $565/0.1 mL + 0.1 mL | $795/0.2 mL + 0.2 mL
Western Blot (WB) (Western blot analysis of extracts from Hela cells using STAT3(Ab-705) Antibody (lane 1)

STAT3, Polyclonal Antibody (Cat# AAA9409580)

Full Name
STAT3 (Ab-705) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB) (Leptin Receptor (LEPR) Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LEPR antibody detected the LEPR protein (arrow).)

Leptin Receptor (LEPR), Polyclonal Antibody (Cat# AAA9204850)

Full Name
Leptin Receptor (LEPR) Antibody (N-term)
Gene Names
LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western Blot analysis of various cells using Stat3 Polyclonal Antibody at dilution of 1:2000.)

Stat3, Polyclonal Antibody (Cat# AAA2535654)

Full Name
Stat3 Polyclonal Antibody
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
Antigen Affinity Purified
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Immunohistochemistry (IHC) (MBS9601062 at 1/200 staining Mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

STAT3, Polyclonal Antibody (Cat# AAA9601062)

Full Name
Phospho-STAT3 (Tyr705) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF/ICC, IP, peptide, EIA
Purity
Peptide affinity purification.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Anti-c-KIT Antibody (N-term) at 1:1000dilution+ Mouse cerebellum tissue lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 110 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

c-KIT, Polyclonal Antibody (Cat# AAA9204642)

Full Name
c-KIT Antibody (N-term)
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human, Monkey
Applications
WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot detection of STAT3 in CHO-K1, C6, 3T3, K562, CEM, Jurkat, Hela, MCF7, COS7, 293T, A431 and A549 cell lysates using STAT3 mouse mAb (1:1000 diluted).Predicted band size:88KDa.Observed band size:88KDa.)

STAT3, Monoclonal Antibody (Cat# AAA475199)

Full Name
Anti-STAT3 Mouse mAb
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat, Monkey, Hamster
Applications
WB
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data (Fig-1: Induction of STAT3 activity by IL-6 in STAT3 Leeporter HEK293 cells.)

STAT3, Cell Line (Cat# AAA668879)

Full Name
STAT3 Leeporter HEK293 Cell Line
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Applications
Functional Assay
Pricing
$4,610/1 Vial | $20,675/5x1 Vial
Immunofluorescence (IF) (Fluorescent confocal image of SY5Y cells stained with phospho-STAT3-S727 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9211779 phospho-STAT3-S727 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-STAT3 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).)

Phospho-STAT3 (S727), Polyclonal Antibody (Cat# AAA9211779)

Full Name
Phospho-STAT3 (S727) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human, mouse
Applications
IF, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot detection of EGFR in HCC827, A549 and MDA-MB-468 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.)

EGF Receptor, Monoclonal Antibody (Cat# AAA475201)

Full Name
Anti-EGF Receptor Mouse mAb
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
WB, ICC, IHC, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Western Blot (WB) (Western blot analysis of STAT3 expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

STAT3, Polyclonal Antibody (Cat# AAA9601869)

Full Name
STAT3 Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot detection of CDK9 in Jurkat, MCF7, C6, 3T3 and Hela cell lysates using CDK9 mouse mAb (1:2000 diluted).Predicted band size:43KDa.Observed band size:43, 50KDa.)

CDK9, Monoclonal Antibody (Cat# AAA476169)

Full Name
Anti-CDK9 Mouse mAb
Gene Names
CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Activity Data (Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.)

Calpain 1, Large Subunit (CAPN1), Active Protein (Cat# AAA2097301)

Full Name
Active Calpain 1, Large Subunit (CAPN1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$220/0.01 mg | $465/0.05 mg | $710/0.1 mg | $880/0.2 mg | $1,715/0.5 mg | $2,555/1 mg | $6,250/5 mg
Western Blot (WB) (Western blot-Phospho-Stat3-Y705 pAb)

Stat3-Y705, Polyclonal Antibody Pair Kit (Cat# AAA9135718)

Full Name
Phospho-Stat3-Y705 Antibody Kit
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB
Pricing
$275/0.02 mL + 0.02 mL | $360/0.05 mL + 0.05 mL | $565/0.1 mL + 0.1 mL | $795/0.2 mL + 0.2 mL
Western Blot (WB) (Anti-CEACAM Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

CEACAM, Polyclonal Antibody (Cat# AAA9204586)

Full Name
CEACAM Antibody (N-term)
Gene Names
CEACAM1; BGP; BGP1; BGPI
Reactivity
Human
Applications
WB, EIA, IHC-P-Leica, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF) (MBS9601378 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

STAT3, Polyclonal Antibody (Cat# AAA9601378)

Full Name
STAT3 Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using STAT3 (Phospho-Ser727) antibody)

STAT3, Polyclonal Antibody (Cat# AAA5309490)

Full Name
STAT3 antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Applications
IF, IHC, WB
Purity
Purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Immunohistochemistry (IHC) (Anti-STAT3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

STAT3, Monoclonal Antibody (Cat# AAA246112)

Full Name
Mouse Monoclonal [clone 7G3H4] (IgG1) to Human STAT3
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human
Applications
IHC - Paraffin, WB, EIA
Purity
Ascites
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Activity Data (Figure. The binding activity of GM-CSF with CSF2Ra.Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. It also has some effects on mature cells of the immune system. These include, for example, inhibiting neutrophil migration and causing an alteration of the receptors expressed on the cells surface. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. Besides, Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra) has been identified as an interactor of GM-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GM-CSF and recombinant rat CSF2Ra. Briefly, GM-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CSF2Ra-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GM-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of GM-CSF and CSF2Ra was shown in Figure 1, and this effect was in a dose dependent manner.)

Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF), Active Protein (Cat# AAA2105061)

Full Name
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)
Gene Names
Csf2; Gmcsf; Gm-csf
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
Immunohistochemistry (IHC) (MBS9601063 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

STAT3, Polyclonal Antibody (Cat# AAA9601063)

Full Name
Phospho-STAT3 (Ser727) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Human Liver)

GATA2, Polyclonal Antibody (Cat# AAA3200601)

Full Name
GATA2 antibody - N-terminal region
Gene Names
GATA2; DCML; IMD21; NFE1B; MONOMAC
Reactivity
Tested Reactivity: Human, Mouse

Predicted Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Protein A purified
Pricing
$390/0.1 mL | $1,750/5x0.1 mL
Western Blot (WB) (Western blot analysis of anti-CSF1R Pab in human placenta. CSF1R (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA9211301)

Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
WB, EIA, FC, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC) (MBS9600211 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

PIAS3, Polyclonal Antibody (Cat# AAA9600211)

Full Name
PIAS3 Antibody
Gene Names
PIAS3; ZMIZ5
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data (Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.)

Calpain 1, Large Subunit (CAPN1), Active Protein (Cat# AAA2097302)

Full Name
Active Calpain 1, Large Subunit (CAPN1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$220/0.01 mg | $465/0.05 mg | $710/0.1 mg | $880/0.2 mg | $1,715/0.5 mg | $2,555/1 mg | $6,250/5 mg
Immunofluorescence (IF) (MBS9601064 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

STAT3, Polyclonal Antibody (Cat# AAA9601064)

Full Name
Phospho-STAT3 (Tyr705) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data (Signal transducer and activator of transcription 3 (STAT3) is a member of the STAT protein family. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. STAT3 is phosphorylated by receptor-associated Janus kinases (JAK), form homo- or heterodimers, and translocate to the cell nucleus where they act as transcription activators. Besides, Epidermal Growth Factor Receptor 2 (EGFR2) has been identified as an interactor of STAT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human STAT3 and recombinant human EGFR2. Briefly, STAT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EGFR2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-STAT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of STAT3 and EGFR2 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of STAT3 with EGFR2)

Signal Transducer And Activator Of Transcription 3 (STAT3), Active Protein (Cat# AAA2105149)

Full Name
Active Signal Transducer And Activator Of Transcription 3 (STAT3)
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$220/0.01 mg | $465/0.05 mg | $715/0.1 mg | $885/0.2 mg | $1,725/0.5 mg | $2,575/1 mg | $6,290/5 mg
Immunohistochemistry (IHC) (Human Uterus)

STAT3, Polyclonal Antibody (Cat# AAA3224461)

Full Name
STAT3 antibody - middle region
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB) (All lanes : Anti-Stat3 Antibody at 1:2000 dilution (upper)Lane 1: HelaLane 2: Hela-Stat3-KnockoutLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 88 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

STAT3, Monoclonal Antibody (Cat# AAA9232247)

Full Name
STAT3 Monoclonal Antibody [Knockout Validated]
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human
Predicted: Mouse, Rat, Pig
Applications
WB, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Immunohistochemistry (IHC) (MBS9602247 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

StIp1, Polyclonal Antibody (Cat# AAA9602247)

Full Name
StIp1 Antibody
Gene Names
ELP2; StIP; MRT58; SHINC-2; STATIP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of STAT3 in human small intestine tissue lysate with STAT3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

STAT3, Polyclonal Antibody (Cat# AAA153378)

Full Name
STAT3 Antibody
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
STAT3 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB) (Western blot analysis of STAT3 expression in K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

STAT3, Polyclonal Antibody (Cat# AAA9601871)

Full Name
STAT3 Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data (Western blot analysis fo extracts from HeLa cells using STAT3 antibody.)

STAT3, Polyclonal Antibody (Cat# AAA5309671)

Full Name
STAT3 antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Applications
IF, IHC, WB
Purity
Purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Western Blot (WB) (Western blot analysis of Phospho-STAT3 (Tyr539) in lysates of LOVO , using Phospho-STAT3 (Tyr539) Antibody.)

STAT3, Polyclonal Antibody (Cat# AAA9610662)

Full Name
Phospho-STAT3 (Tyr539) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Sheep, Rabbit, Chicken
Applications
WB, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western BlotPositive WB detected in?A549 whole cell lysate(treated with EGF or not)All lanes?Phospho-STAT3 antibody at 1.88ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 98 KDaObserved band size: 98 KDa)

STAT3, Monoclonal Recombinant Antibody (Cat# AAA7114904)

Full Name
Phospho-STAT3 (S727) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human
Applications
EIA, WB, IHC, IP
Purity
Affinity-Chromatography
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL

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