Immunohistochemistry (IHC)
(MBS9602247 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit StIp1 Polyclonal Antibody | anti-StIp1 antibody
StIp1 Antibody
Gene Names
ELP2; StIP; MRT58; SHINC-2; STATIP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
StIp1; Polyclonal Antibody; StIp1 Antibody; AU023723; Elongation protein 2 homolog (S. cerevisiae); Elongator acetyltransferase complex subunit 2; Elongator complex protein 2; elongator protein 2; ELP2; ELP2_HUMAN; Epl2; FLJ10879; SHINC 2; SHINC-2; SHINC2; signal transducer and activator of transcription 3 interacting protein 1; signal transducer and activator of transcription interacting protein 1; STAT3 interacting protein 1; Stat3 interacting protein; STAT3-interacting protein 1; STATIP 1; STATIP1; STATIP1 protein; StIP; StIP1; anti-StIp1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
StIp1 antibody detects endogenous levels of total StIp1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
891
Applicable Applications for anti-StIp1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IHC: 1:200
IF/ICC: 1:100-1:500
IHC: 1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human StIp1
Subcellular Location
Cytoplasm. Nucleus.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (89%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9602247 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9602247 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis StIp1 using HT29 whole cell lysates)
Western Blot (WB)
(Western blot analysis StIp1 using HT29 whole cell lysates)
Immunofluorescence (IF)
(MBS9602247 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9602247 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-StIp1 antibody
Function: Regulates the ligand-dependent activation of STAT3.
Subunit Structure: Interacts with STAT3 and JAKs (By similarity). Component of the RNA polymerase II elongator complex (Elongator), which consists of ELP1, STIP1/ELP2, ELP3, ELP4, and two yet unidentified proteins, p30 and p38. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit.
Similarity: Belongs to the WD repeat ELP2 family.
Subunit Structure: Interacts with STAT3 and JAKs (By similarity). Component of the RNA polymerase II elongator complex (Elongator), which consists of ELP1, STIP1/ELP2, ELP3, ELP4, and two yet unidentified proteins, p30 and p38. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit.
Similarity: Belongs to the WD repeat ELP2 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 93 kDa
Predicted: 93 kDa
Predicted: 93 kDa
NCBI Official Full Name
elongator complex protein 2 isoform 1
NCBI Official Synonym Full Names
elongator acetyltransferase complex subunit 2
NCBI Official Symbol
ELP2
NCBI Official Synonym Symbols
StIP; MRT58; SHINC-2; STATIP1
NCBI Protein Information
elongator complex protein 2
UniProt Protein Name
Elongator complex protein 2
Protein Family
UniProt Gene Name
ELP2
UniProt Synonym Gene Names
STATIP1; ELP2; StIP1
NCBI Description
The protein encoded by this gene is a core subunit of the elongator complex, a histone acetyltransferase complex that associates with RNA polymerase II. In addition to histone acetylation, the encoded protein effects transcriptional elongation and may help remodel chromatin. [provided by RefSeq, May 2016]
Uniprot Description
Regulates the ligand-dependent activation of STAT3.
Research Articles on StIp1
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Product Notes
The StIp1 elp2 (Catalog #AAA9602247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The StIp1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's StIp1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IHC: 1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the StIp1 elp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "StIp1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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