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SDS-Page

ATP5O recombinant protein

ATP5O, 24-213aa, Human, His tag, E Coli

Gene Names
ATP5PO; ATPO; OSCP; ATP5O; HMC08D05
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
ATP5O; 24-213aa; Human; His tag; E Coli; ATP synthase subunit O; ATPO; OSCP; ATP5O recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MFAKLVRPPV QVYGIEGRYA TALYSAASKQ NKLEQVEKEL LRVAQILKEP KVAASVLNPY VKRSIKVKSL NDITAKERFS PLTTNLINLL AENGRLSNTQ GVVSAFSTMM SVHRGEVPCT VTSASPLEEA TLSELKTVLK SFLSQGQVLK LEAKTDPSIL GGMIVRIGEK YVDMSVKTKI QKLGRAMREI V
Sequence Length
213
Applicable Applications for ATP5O recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for ATP5O recombinant protein
ATP synthase subunit O, also known as ATP5O, localizes to the mitochondria and catalyzes ATP synthesis. The protein is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance. Recombinant human ATP5O protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for ATP5O recombinant protein
References
Hundal T., et al. (1984) J Bioenerq Biomembr. 16:535-550.; Joshi S., et al. (1992) J Biol Chem. 267:12860-12867.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
539
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23.1 kDa (211aa), confirmed by MALDI-TOF
NCBI Official Full Name
ATP synthase subunit O, mitochondrial
NCBI Official Synonym Full Names
ATP synthase peripheral stalk subunit OSCP
NCBI Official Symbol
ATP5PO
NCBI Official Synonym Symbols
ATPO; OSCP; ATP5O; HMC08D05
NCBI Protein Information
ATP synthase subunit O, mitochondrial
UniProt Protein Name
ATP synthase subunit O, mitochondrial
UniProt Gene Name
ATP5O
UniProt Synonym Gene Names
ATPO; OSCP

NCBI Description

The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance. [provided by RefSeq, Jul 2008]

Uniprot Description

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements.

Research Articles on ATP5O

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Product Notes

The ATP5O atp5o (Catalog #AAA204007) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's ATP5O can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the ATP5O atp5o for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MFAKLVRPPV QVYGIEGRYA TALYSAASKQ NKLEQVEKEL LRVAQILKEP KVAASVLNPY VKRSIKVKSL NDITAKERFS PLTTNLINLL AENGRLSNTQ GVVSAFSTMM SVHRGEVPCT VTSASPLEEA TLSELKTVLK SFLSQGQVLK LEAKTDPSIL GGMIVRIGEK YVDMSVKTKI QKLGRAMREI V. It is sometimes possible for the material contained within the vial of "ATP5O, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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