STAT3 elisa kit

Phospho-STAT3 (Tyr705) Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9501569

• Gene Names: STAT3; APRF; HIES; ADMIO; ADMIO1
• Reactivity: Human, Mouse, Rat
• Synonyms: STAT3; Phospho-STAT3 (Tyr705) Colorimetric Cell-Based ELISA Kit; Signal transducer and activator of transcription 3; Acute-phase response factor; APRF; STAT3 elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: The Anti-STAT3 (Phospho-Tyr705) Antibody is a rabbit polyclonal antibody. It was tested on Western Blots for specificity. The data in Figure 2 shows that a single protein band was detected. This protein band can be blocked by the synthesized immunogen peptide.
• Form/Format: Two 96-Well Plates
• Sequence Length: 769

• Preparation and Storage: Store at 4 degree C.

Related Product Information for STAT3 elisa kit

Principle of the Assay: The STAT3 (Phospho-Tyr705) Cell-Based ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can monitor STAT3 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated STAT3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on STAT3 phosphorylation.

Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the nonphosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Product Categories/Family for STAT3 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 21618338
• NCBI GeneID: 6774
• NCBI Accession #: NP_003141.2
• NCBI GenBank Nucleotide #: NM_003150.3
• UniProt Accession #: P40763; O14916; Q9BW54; A8K7B8; K7ENL3
• Molecular Weight: 88068
• NCBI Official Full Name: signal transducer and activator of transcription 3 isoform 2
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3
• NCBI Official Symbol: STAT3
• NCBI Official Synonym Symbols: APRF; HIES; ADMIO; ADMIO1
• NCBI Protein Information: signal transducer and activator of transcription 3
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT3

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; cytosol; mitochondrial inner membrane; nuclear chromatin; nucleoplasm; nucleus; plasma membrane; RNA polymerase II transcription factor complex

Molecular Function: CCR5 chemokine receptor binding; chromatin DNA binding; DNA binding; DNA binding transcription factor activity; glucocorticoid receptor binding; identical protein binding; ligand-dependent nuclear receptor activity; protein binding; protein dimerization activity; protein homodimerization activity; protein kinase binding; protein phosphatase binding; signal transducer activity; transcription factor binding; transcription regulatory region DNA binding

Biological Process: acute-phase response; aging; astrocyte differentiation; cell motility; cell proliferation; cellular response to hormone stimulus; cellular response to leptin stimulus; cellular response to organic cyclic compound; cytokine-mediated signaling pathway; eating behavior; eye photoreceptor cell differentiation; glucose homeostasis; inflammatory response; interleukin-6-mediated signaling pathway; interleukin-7-mediated signaling pathway; intracellular receptor signaling pathway; JAK-STAT cascade; leptin-mediated signaling pathway; miRNA mediated inhibition of translation; mRNA transcription from RNA polymerase II promoter; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of glycolytic process; negative regulation of hydrogen peroxide biosynthetic process; negative regulation of neuron death; negative regulation of neuron migration; negative regulation of stem cell differentiation; negative regulation of transcription from RNA polymerase II promoter; nervous system development; phosphorylation; positive regulation of angiogenesis; positive regulation of ATP biosynthetic process; positive regulation of erythrocyte differentiation; positive regulation of gene expression; positive regulation of gene silencing by miRNA; positive regulation of growth factor dependent skeletal muscle satellite cell proliferation; positive regulation of metalloendopeptidase activity; positive regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of tyrosine phosphorylation of STAT protein; positive regulation of vascular endothelial cell proliferation; protein import into nucleus; radial glial cell differentiation; regulation of cell cycle; regulation of mitochondrial membrane permeability; regulation of multicellular organism growth; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to drug; response to estradiol; response to ethanol; response to leptin; sexual reproduction; signal transduction; somatic stem cell maintenance; T-helper 17 cell lineage commitment; thermoregulation; viral process

Disease: Autoimmune Disease, Multisystem, Infantile-onset; Hyper-ige Recurrent Infection Syndrome, Autosomal Dominant