Western Blot (WB)
(Anti-PNMT Antibody (Center)at 1:2000 dilution + SW620 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human PNMT Polyclonal Antibody | anti-PNMT antibody
PNMT Antibody (Center)
Western Blot (WB)
(Anti-PNMT Antibody (Center)at 1:2000 dilution + SW620 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-PNMT Antibody (Center)at 1:2000 dilution + SW620 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Sasaoka T.,et al.Neurochem. Int. 15:555-565(1989).
Baetge E.E.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:3648-3652(1988).
Martin J.L.,et al.Structure 9:977-985(2001).
Gee C.L.,et al.Biochemistry 44:16875-16885(2005).
NCBI and Uniprot Product Information
NCBI Description
The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
Uniprot Description
PNMT: Converts noradrenaline to adrenaline. Belongs to the NNMT/PNMT/TEMT family.
Protein type: Amino Acid Metabolism - tyrosine; EC 2.1.1.28; Methyltransferase
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: cytosol
Molecular Function: phenylethanolamine N-methyltransferase activity
Biological Process: methylation; catecholamine biosynthetic process; epinephrine biosynthetic process
Disease: Hypertension, Essential
Research Articles on PNMT
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Product Notes
The PNMT pnmt (Catalog #AAA9202041) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PNMT Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PNMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the PNMT pnmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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