Testing Data
(Whole cell lysate from human HEK293 (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells using MBS601404. Predicted molecular weight: 273kD)
Mouse PRP8 Pre-mRNA Processing Factor 8 Homolog Monoclonal Antibody | anti-PRPF8 antibody
PRP8 Pre-mRNA Processing Factor 8 Homolog (PRPF8, PRP8, hPRP8, PRPC8, RP13)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Western Blot: 0.2-2ug/ml
Immunocytochemistry: 2-100ug/ml
Flow Cytometry: 0.5-2ug/sample
Testing Data
(Whole cell lysate from human HEK293 (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells using MBS601404. Predicted molecular weight: 273kD)
Testing Data
(Whole cell lysate from human HEK293 (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells using MBS601404. Predicted molecular weight: 273kD)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with MBS601404 (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with MBS601404 (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS601404.)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS601404.)
NCBI and Uniprot Product Information
NCBI Description
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq]
Uniprot Description
PRPF8: Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes. Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.
Protein type: Spliceosome; RNA-binding; RNA splicing
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: nucleoplasm; membrane; snRNP U5; nuclear speck; nucleus
Molecular Function: U5 snRNA binding; protein binding; U6 snRNA binding
Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; assembly of spliceosomal tri-snRNP; RNA splicing; gene expression; mRNA processing
Disease: Retinitis Pigmentosa 13
Research Articles on PRPF8
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Product Notes
The PRPF8 prpf8 (Catalog #AAA601404) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PRP8 Pre-mRNA Processing Factor 8 Homolog (PRPF8, PRP8, hPRP8, PRPC8, RP13) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRP8 Pre-mRNA Processing Factor 8 Homolog can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Gel Shift Assay (GS/EMSA). Suitable for use in Western Blot, Dot Blot, Immunocytochemistry and Flow Cytometry. Dilution: Western Blot: 0.2-2ug/ml Immunocytochemistry: 2-100ug/ml Flow Cytometry: 0.5-2ug/sample. Researchers should empirically determine the suitability of the PRPF8 prpf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRP8 Pre-mRNA Processing Factor 8 Homolog, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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